CbAnoAest
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB97985 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Anoectangium aestivum (summer-moss), genomic and transcriptomic data
| PRJEB97984 | ENA
Project description:High group animals with supercooling point (SCP) above -15°C and selected low group animals with SCP below -15°C were prepared from Cryptopygus antarcticus collected from wet moss (Sanionia uncinata (Hedw.)) during the austral summer of 2005 at the British Antarctic Survey's research station at Rothera Point, Adelaide Island (67'34'S, 66'8'W).
2008-09-01 | E-MEXP-1569 | biostudies-arrayexpress
Project description:Analysis of transcriptome in moss Physcomitrella patens CNGCb null mutant at 25 and 34 degrees C for 30 minutes. Results provide insight into role of CNGCb in acquired thermotolerance induced by non-lethal heat treatment. Typically at dawn of a hot summer day, land plants need precise molecular thermometers to sense harmless increments in the ambient temperature to timely develop a heat-shock response (HSR) and accumulate protective heat shock proteins (Hsps), in anticipation of upcoming harmful temperatures at mid-day. Here, we found that the CNGCb gene from Physcomitrella patens and its Arabidopsis ortholog CNGC2, encode for a component of cyclic nucleotide gated Ca2+ channels acting as the primary thermosensors of land plant cells. Disruption of CNGCb or CNGC2 produced a hyper-thermosensitive phenotype, giving rise to a HSR and acquired thermotolerance at significantly milder heat-priming treatments than in wild type plants. In an aequorin-expressing moss, CNGCb loss-of-function caused altered Ca2+ signaling and a sustained Ca2+ influx. Patch clamp recordings on moss protoplasts showed the presence of three distinct thermo-responsive Ca2+-channels in wild type cells. Deletion of CNGCb led to a total absence of one, and it increased the open probability of the remaining two thermo-responsive Ca2+ channels. Thus, both in Arabidopsis and moss, CNGC2 and CNGCb are expected to form with other related CNGCs, heteromeric Ca2+ channels in the plasma membrane that respond to mild increments in the ambient temperature by triggering an optimal HSR, leading to the onset of plant acquired thermotolerance.
2012-03-06 | GSE36301 | GEO
Project description:Analysis of transcriptome in moss Physcomitrella patens CNGCb null mutant at 25 and 34 degrees C for 30 minutes. Results provide insight into role of CNGCb in acquired thermotolerance induced by non-lethal heat treatment. Typically at dawn of a hot summer day, land plants need precise molecular thermometers to sense harmless increments in the ambient temperature to timely develop a heat-shock response (HSR) and accumulate protective heat shock proteins (Hsps), in anticipation of upcoming harmful temperatures at mid-day. Here, we found that the CNGCb gene from Physcomitrella patens and its Arabidopsis ortholog CNGC2, encode for a component of cyclic nucleotide gated Ca2+ channels acting as the primary thermosensors of land plant cells. Disruption of CNGCb or CNGC2 produced a hyper-thermosensitive phenotype, giving rise to a HSR and acquired thermotolerance at significantly milder heat-priming treatments than in wild type plants. In an aequorin-expressing moss, CNGCb loss-of-function caused altered Ca2+ signaling and a sustained Ca2+ influx. Patch clamp recordings on moss protoplasts showed the presence of three distinct thermo-responsive Ca2+-channels in wild type cells. Deletion of CNGCb led to a total absence of one, and it increased the open probability of the remaining two thermo-responsive Ca2+ channels. Thus, both in Arabidopsis and moss, CNGC2 and CNGCb are expected to form with other related CNGCs, heteromeric Ca2+ channels in the plasma membrane that respond to mild increments in the ambient temperature by triggering an optimal HSR, leading to the onset of plant acquired thermotolerance. The WT moss tissues were heat-shocked for a half an hour at 34°C and 38°C and CNGCb at 25and 34°C followed by liquid nitrogen freezing. Total RNA was isolated using RNeasy Mini Kit (QIAGEN, Hilden, Germany) and two biological replicate samples for each treatment, were extracted. An Agilent-certified microarray service lab (MOgene, LC, St. Louis, MO, USA) was used to verify the integrity of the RNA and perform the microarray experiments. Two biological replicates were performed.
2012-03-06 | E-GEOD-36301 | biostudies-arrayexpress
Project description:High-throughput sequencing of endogenous small RNAs from the moss Physcomitrella patens. This dataset encompasses microRNAs and other small RNAs of ~20-24 nucleotides expressed in the moss P. patens. SAMPLES UPDATED JULY 9, 2007 TO INCLUDE DATA ON SEQUENCED SMALL RNAS THAT DO NOT MATCH THE P. PATENS GENOME Keywords: High throughput small RNA sequencing
2006-08-15 | GSE5103 | GEO
Project description:4plex_physco_2014-05 - ppmax2 response to gr24 - How does the Ppmax2 moss mutant respond to Strigolactone (GR24)? - Two moss genotypes are used: WT and the Ppmax2 mutant. Moss tissues are fragmented, then plated on medium (Petri dish with cellophane disks) and cultivated for 3 weeks. Moss tissues are then transfered for 6 hours on acetone-containing medium (control treatment, for WT and Ppmax2) or GR24 (1 microM, in acetone)-containing medium (for Ppmax2). After 6 hours, the moss tissues are collected, quickly forzen in liquid nitrogen. RNA are isolated using the Quiagen RNeasy Plant mini kit (including a RNase-free DNase treatment on column). Two similar experiments (T1 and T2) have been led.
2017-04-22 | GSE80578 | GEO
Project description:To examine the difference of the endometrial conditions in the cows between on summer and autumn seasons, gene expression profiles were compared. The expressions of 268 genes were significantly higher in the endometrium collected on summer than those on autumn, whereas 369 genes were lower (P<0.05 or lower). Transcripts of GP2 and NTS were more abundant in the endometrium of summer than those of autumn (P<0.05). In contrast, the mRNA expressions of CDH1 and HSPH1 were lower in the endometrium of summer seasons (P<0.1). Collectively, the different gene expression profiles may contribute to functional differences of endometrium between the summer and autumn seasons, and GP2 and NTS may have a relevance to endometrial deficiency that cause infertility of cows on summer seasons.
2015-02-19 | GSE66057 | GEO
Project description:Comparison of gene expression levels in non-hardened (NH), cold-hardened (CH) and subzero-hardened (SZH) Triticum aestivum L. cv Jackson crowns with Affymetrix GeneChip Barley Genome Array. Keywords = wheat Keywords = crowns Keywords = cold tolerance Keywords = freezing resistance Keywords = Triticum aestivum L. cv Jackson Keywords: repeat sample
2005-01-20 | GSE2166 | GEO
Project description:One day cold (14 and 19 °C) and hydrogen peroxide (H2O2) treatment of wheat (Triticum aestivum ssp. aestivum L.) variety Chinese Spring and two chromosome 5A substitution lines of Chinese Spring, Chinese Spring(T. ae. ssp. aestivum L. Cheyenne 5A) and Chinese Spring(T. ae. ssp. spelta L. 5A).
2018-09-28 | E-MTAB-6627 | biostudies-arrayexpress