Project description:RNA-seq was performed to examine the differential expressed transcriptomes with five-point experiment (6, 12, 24, 48 and 96 h) at the stem bases of cuttings in PuHox52 overexpression line compare to wild type Populus ussuriensis.
Project description:Drought is one of the major environmental problem in terms of limiting the survival of plant. Roots are the first plant organ to sense drought stress, so it is important to understanding of the molecular machanisms of drought stress responses in the roots. Here we aim to characterized the gene expression profile in Populus ussuriensis roots at 0, 6, 12, 24, 48 and 120 h after the start of PEG-induced drought stress. A total of 2 μg RNA per sample was used to generate sequencing libraries using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA). Libraries were sequenced in 150 bp paired-end mode, using an Illumina HiSeq X Ten platform. Our results provide a global view of gene expression profile that contributes to drought resistance in Populus ussuriensis, and meaningful information for genetic engineering research in the future.
Project description:One week-old in-vitro plants of PuCH35 overexpression (PuC3H35-OE) lines and wildtype (WT) Populus ussuriensis were transfered to 1/2 MS medium supplemented with or without 6% PEG6000 for 7 days, and the root tips (0.5 cm long) were harvested. Samples were collected from three biological replicates (25 in-vitro plants each).
Project description:Illumina technology was used to generate mRNA profiles of Populus tremula x alba 717-1B4 control roots and Laccaria bicolor S238N ectomycorrhiza. Total RNA was extracted, TruSeq mRNA Stranded libraries were constructed and and sequenced (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the Populus trichocarpa v4.1 primary transcripts available at Phytozome (https://phytozome-next.jgi.doe.gov/info/Ptrichocarpa_v4_1l) using CLC Genomics Workbench v24.
