Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Profile of mRNA bound to circular RNA BTBD7_hsa_circ_0000563 in coronary artery disease investigated via chromatin isolation by RNA purification followed by RNA sequencing

ABSTRACT: Profile of mRNA bound to circular RNA BTBD7_hsa_circ_0000563 in coronary artery disease investigated via chromatin isolation by RNA purification followed by RNA sequencing

PROVIDER: PRJNA1000458 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25463551_1.fastq.gz	Fastqsanger.gz
	SRR25463551_2.fastq.gz	Fastqsanger.gz
	SRR25463552_1.fastq.gz	Fastqsanger.gz
	SRR25463552_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Profile of mRNA bound to circular RNA BTBD7_hsa_circ_0000563 in coronary artery disease investigated via chromatin isolation by RNA purification followed by RNA sequencing

Project description:Background: As a novel circRNA, BTBD7_hsa_circ_0000563 has not been fully investigated in coronary artery disease (CAD). Our aim is to reveal the possible functional role and regulatory pathway of BTBD7_hsa_circ_0000563 in CAD via exploring genes combined with BTBD7_hsa_circ_0000563. Methods: A total of 45 peripheral blood mononuclear cell (PBMC) samples of CAD patients were enrolled. The ChIRP-RNAseq assay was performed to directly explore genes bound to BTBD7_hsa_circ_0000563. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to reveal possible functions of these genes. The interaction network was constructed by the STRING database and the Cytoscape software. The Cytoscape software were used again to identify clusters and hub genes of genes bound to BTBD7_hsa_circ_0000563. The target miRNAs of hub genes were predicted via online databases. Results: In this study, a total of 221 mRNAs directly bound to BTBD7_hsa_circ_0000563 were identified in PBMCs of CAD patients via ChIRP-RNAseq. The functional enrichment analysis revealed that these mRNAs may participate in translation and necroptosis. Moreover, the interaction network showed that there may be a close relationship between these mRNAs. Eight clusters can be further subdivided from the interaction network. RPS3 and RPSA were identified as hub genes and hsa-miR-493-5p was predicted to be the target miRNA of RPS3. Conclusions: BTBD7_hsa_circ_0000563 and mRNAs directly bound to it may influence the initiation and progression of CAD, among which RPS3 and RPSA may be hub genes. These findings may provide innovative ideas for further research on CAD.

2024-01-31 | GSE239635 | GEO

Purification-sequencing of RNA super-assemblies.

Project description:Purification-sequencing of RNA super-assemblies.

| PRJNA879772 | ENA

Chromatin Isolation by RNA Purification (ChIRP) assays followed by LC-MS in DCRT-KO cell lines

Project description:To explore the molecular mechanism of DCRT in DCM, Chromatin Isolation by RNA Purification (ChIRP) assays followed by Liquid Chromatography Mass Spectrometry (LC-MS) analyses were conducted. Each probe was designed using the web-based designer provided by Sangon Biotech, biotin-TEG-labeled separated into odd and even groups. Probes that targeted LacZ were utilized as a safeguard. For each sample, 4 × 10^7 WT or DCRT-KO AC16 cells were used and crosslinked in 1% glutaraldehyde at room temperature for 10 min, and quenched with 125 mM glycine for 5 min. Cleared lysates were hybridized with probes in 37 °C in a hybridization oven for 4 h. After washes and elution, proteins were purified and LC-MS

| PXD046020 |

Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

Project description:We provide a method for high quality RNA purification out of a small number (5000 - 100,000) of FACS sorted zebrafish cells followed by RNA sequencing

2019-03-11 | GSE121917 | GEO

RNA Affinity Purification followed by sequencing (RAP-seq) allows identification of transcriptome-wide RBP binding sites using recombinant RBPs and purified total cellular RNA

Project description:RNA Affinity Purification followed by sequencing (RAP-seq) allows identification of transcriptome-wide RBP binding sites using recombinant RBPs and purified total cellular RNA

| PRJEB92198 | ENA

Identification of ECM3 interactors by affinity purification followed by mass spectrometry analysis.

Project description:Identification of ECM3 interactors by affinity purification followed by mass spectrometry analysis.

2022-11-03 | PXD017773 | Pride

Defining the RNA Interactome by Total RNA-Associated Protein Purification

Project description:Defining the RNA Interactome by Total RNA-Associated Protein Purification

| PRJNA490515 | ENA

RNA-bound proteome of Mycobacterium bovis

Project description:The dataset accompanying a publication describing the RNA degradosome components in Mycobacterium tuberculosis (Mtb). The RNA-bound proteome was determined by affinity purification of protein-RNA complexes from Mycobacterium bovis cells fed with 4-thiouridine and crosslinked via photoactivation at 365nm. Affinity purified RNA-protein complexes were subjected to LC-MS analysis

2019-04-01 | PXD012661 | Pride

MS2-affinity purification coupled with RNA sequencing (MAPS) reveals S. aureus RsaG sRNA targetome

Project description:MS2-affinity purification coupled with RNA sequencing (MAPS) reveals S. aureus RsaG sRNA targetome. Affinity purification of in vivo regulatory complexes coupled with high throughput RNA sequencing methodology or MAPS standing for “MS2 affinity purification coupled to RNA".

2024-06-01 | GSE176028 | GEO

AKAP12 UV RNA immunoprecipitation followed by high throughput sequencing

Project description:AKAP12 UV RNA immunoprecipitation followed by high throughput sequencing

| PRJNA1040162 | ENA