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Polyadenylation of mRNAs encoding secreted proteins by TENT5 family of enzymes is essential for gametogenesis in mice

ABSTRACT: Polyadenylation of mRNAs encoding secreted proteins by TENT5 family of enzymes is essential for gametogenesis in mice

PROVIDER: PRJNA1000501 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25470102_1.fastq.gz	Fastqsanger.gz
	SRR25470102_2.fastq.gz	Fastqsanger.gz
	SRR25470103_1.fastq.gz	Fastqsanger.gz
	SRR25470103_2.fastq.gz	Fastqsanger.gz
	SRR25470104_1.fastq.gz	Fastqsanger.gz

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Polyadenylation of mRNAs encoding secreted proteins by TENT5 family of enzymes is essential for gametogenesis in mice

Project description:Cytoplasmic polyadenylation plays a vital role in gametogenesis, however, the participating enzymes and substrates in mammals remain unclear. Using knockout and knock-in mouse models, we describe the essential role of 4 TENT5 poly(A) polymerases in mice fertility and gametogenesis. TENT5B and TENT5C play crucial, but redundant roles in oogenesis, with double knockout of both genes leading to oocyte degeneration. Additionally, TENT5B-GFP females display gain-of-function infertility effect with multiple chromosomal aberrations in ovulated oocytes. TENT5C and TENT5D both regulate different stages of spermatogenesis, shown by sterility of males with either gene’s knockout mutation. Finally, Tent5a knockout significantly lowers fertility, although the underlying mechanism is not directly related to gametogenesis. Through Direct RNA sequencing we discovered that TENT5 proteins polyadenylate mRNAs encoding endoplasmic reticulum-targeted proteins essential for gametogenesis. Sequence motives analysis and reporter mRNA assay revealed that the presence of endoplasmic reticulum-leader represents the primary determinant of TENT5-mediated regulation.

2024-05-16 | GSE239661 | GEO

TENT5-mediated polyadenylation of mRNAs encoding secreted proteins is essential for gametogenesis in mice.

Project description:Cytoplasmic polyadenylation plays a vital role in gametogenesis; however, the participating enzymes and substrates in mammals remain unclear. Using knockout and knock-in mouse models, we describe the essential role of four TENT5 poly(A) polymerases in mouse fertility and gametogenesis. TENT5B and TENT5C play crucial yet redundant roles in oogenesis, with the double knockout of both genes leading to oocyte degeneration. Additionally, TENT5B-GFP knock-in females display a gain-of-function infertility effect, with multiple chromosomal aberrations in ovulated oocytes. TENT5C and TENT5D both regulate different stages of spermatogenesis, as shown by the sterility in males following the knockout of either gene. Finally, Tent5a knockout substantially lowers fertility, although the underlying mechanism is not directly related to gametogenesis. Through direct RNA sequencing, we discovered that TENT5s polyadenylate mRNAs encoding endoplasmic reticulum-targeted proteins essential for gametogenesis. Sequence motif analysis and reporter mRNA assays reveal that the presence of an endoplasmic reticulum-leader sequence represents the primary determinant of TENT5-mediated regulation.

| S-EPMC11193744 | biostudies-literature

Induction of genes encoding lignocellulolytic enzymes in the basidiomycete Dichomitus squalens

Project description:Induction of genes encoding lignocellulolytic enzymes in the basidiomycete Dichomitus squalens

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Induction of genes encoding lignocellulolytic enzymes in the basidiomycete Dichomitus squalens strain FBCC312

Project description:Expression of genes encoding plant biomass degrading enzymes in ascomycetes is controlled by transcriptional activators that respond to the presence of monomeric components of plant polymers. It can be expected that a similar system exists in basidiomycetes, but no homologs of the ascomycete regulators could be identified in basidiomycetes.

2018-04-05 | GSE98345 | GEO

Control of secreted protein gene expression and the mammalian secretome by the metabolic regulator PGC-1a

Project description:Secreted proteins serve pivotal roles in the development of multicellular organisms, acting as structural matrix, extracellular enzymes and signal molecules. In this study we demonstrate, unexpectedly, that PGC-1α, a critical transcriptional co-activator of metabolic gene expression, functions to down-regulate expression of diverse genes encoding secreted molecules and extracellular matrix (ECM) components to modulate the secretome. We show that both endogenous and exogenous PGC-1α down-regulate expression of numerous genes encoding secreted molecules. Mechanistically, results obtained using mRNA stability measurements as well as intronic RNA expression analysis are consistent with a transcriptional effect of PGC-1α on expression of genes encoding secreted proteins. Interestingly, PGC-1α requires the central heat shock response regulator HSF1 to affect some of its targets, and both factors co-reside on several target genes encoding secreted molecules in cells. Finally, using a mass spectrometric analysis of secreted proteins, we demonstrate that PGC-1α modulates the secretome of mouse embryonic fibroblasts (MEFs).

2016-12-01 | GSE87100 | GEO

Induction of genes encoding lignocellulolytic enzymes in the basidiomycete monokaryotic Dichomitus squalens strain CBS 464.89

2018-04-05 | GSE105023 | GEO

Induction of genes encoding lignocellulolytic enzymes in the basidiomycete monokaryotic Dichomitus squalens strain CBS 464.89

Project description:Induction of genes encoding lignocellulolytic enzymes in the basidiomycete monokaryotic Dichomitus squalens strain CBS 464.89

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A tomato fruit RNA extraction method that isolates mRNAs encoding secreted and endomembrane associated proteins

Project description:Background: During the analysis of ripening related gene expression in tomato fruit, we observed a bias towards certain classes of messenger RNA based upon the type of buffer used in the initial step of the extraction procedure. We postulated that there was a functional association of the separated transcripts. To test this hypothesis, we carried out extractions from the same tissue sample where only the buffer varied. Transcripts were hybridised to Affymetrix oligo arrays and the data was analysed according to the predicted cellular component of the encoded proteins. Results: The use of an extraction buffer that lacked high levels of caeotropic agents resulted in a reduction of mRNAs encoding proteins that were either secreted or were predicted to be routed through the endomembrane system and hence could have been bound to the endoplasmic reticulum in polyribosomes. Extraction of the cell debris immediately following the initial buffer based extraction and subsequent micro array analysis revealed that the expected transcripts could be recovered. This demonstrated that the buffer was separating the mRNAs based on the cellular component of the encoded proteins. Analysis of selected transcripts by northern hybridisation again supported this theory. Conclusions: Some traditional buffers used for fruit RNA extraction selectively deplete for transcripts encoding proteins that are membrane-associated or secreted. This can be explained if polyribosomes that are bound to the endoplasmic reticulum (ER) are not effectively disrupted when the extraction buffer lacks either detergent or organic solvents. These findings have important implications with respect to experimental bias, as well as opportunities for message enrichment and protein characterisation. GeneChip analyses were performed to analyse the effect of using different extraction protocols on Solanum lycopersicum pericarp.

2011-01-21 | E-GEOD-17222 | biostudies-arrayexpress

Competition or indifference: Neutron-Encoding to profile linkage selectivity of deubiquitinating enzymes

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2022-10-05 | MSV000090455 | MassIVE

Saccharina latissima gametogenesis

Project description:Male and female gametogenesis in the kelp Saccharina latissima (Laminariales, Laminariaceae)

| PRJEB32911 | ENA