Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Aedes aegypti NeSt1 interacts with human CD47 to enhance Zika virus infectivity

ABSTRACT: Aedes aegypti NeSt1 interacts with human CD47 to enhance Zika virus infectivity

PROVIDER: PRJNA1001044 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25487800_1.fastq.gz	Fastqsanger.gz
	SRR25487800_2.fastq.gz	Fastqsanger.gz
	SRR25487801_1.fastq.gz	Fastqsanger.gz
	SRR25487801_2.fastq.gz	Fastqsanger.gz
	SRR25487802_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 128

Similar Datasets

Aedes aegypti NeSt1 interacts with human CD47 to enhance Zika virus infectivity

Project description:Aedes aegypti is a vector of many infectious agents, including flaviviruses like Zika virus. We demonstrate that Nest1, a 34kDa mosquito salivary protein, facilitates Zika virus dissemination in human skin explants. Our aim was to analyze the effect of Nest1 at a transcriptomic level (RNAseq) in human skin explants, in the presence and absence of virus (ZIKV), at different timepoints (day 1, 2,3 ,and 4)

2024-12-05 | GSE239840 | GEO

Cd47

Project description:Rattus norvegicus Cd47, Cd47 molecule [Source:RGD Symbol;Acc:2308], is differentially expressed in 32 experiment(s);

| ENSRNOG00000001964 | ExpressionAtlas

Cd47

Project description:Rattus norvegicus Cd47, Cd47 molecule [Source:RGD Symbol;Acc:2308], is expressed in 4 baseline experiment(s);

| ENSRNOG00000001964 | ExpressionAtlas

CD47

Project description:Monodelphis domestica CD47, CD47 molecule [Source:NCBI gene;Acc:100009991], is expressed in 2 baseline experiment(s);

| ENSMODG00000018045 | ExpressionAtlas

Differentiation enhances Zika virus infection in neuronal brain cells

Project description:Zika virus (ZIKV) is an emerging, mosquito-borne pathogen associated with a widespread 2015–2016 epidemic in the Western Hemisphere and a proven cause of microcephaly and other fetal brain defects in infants born to infected mothers. ZIKV infections have been also linked to other neurological illnesses in infected adults and children, including Guillain-Barré syndrome (GBS), acute flaccid paralysis (AFP) and meningoencephalitis, but the viral pathophysiology behind those conditions remains poorly understood. Here we investigated ZIKV infectivity in neuroblastoma SH-SY5Y cells, both undifferentiated and following differentiation with retinoic acid. We perform RNA seq, and global trancriptome analysis to corroborate the effect of retinoic acid in SH-SY5Y cells. Then we analyze the virus infection in differentiated and undifferntiated cells. We found that multiple ZIKV strains, representing both the prototype African and contemporary Asian epidemic lineages, were able to replicate in SH-SY5Y cells. Differentiation with resultant expression of mature neuron markers increased infectivity in these cells, and the extent of infectivity correlated with degree of differentiation. Enhanced ZIKV infectivity in a neural cell line following differentiation may contribute to viral neuropathogenesis in the developing or mature central nervous system.

2018-10-20 | GSE121534 | GEO

Expression data comparing Huh7 control and CD47 knockdown cells

Project description:To identify candidate genes regulated by CD47 Affymetrix Human Genome U133 Plus GeneChip 2.0 HCC cell line Huh7 cells with or without CD47 repressed

2014-06-01 | E-GEOD-47563 | biostudies-arrayexpress

Evolutionary enhancement of Zika virus infectivity in Aedes aegypti mosquitoes.

Project description:Zika virus (ZIKV) remained obscure until the recent explosive outbreaks in French Polynesia (2013-2014) and South America (2015-2016). Phylogenetic studies have shown that ZIKV has evolved into African and Asian lineages. The Asian lineage of ZIKV was responsible for the recent epidemics in the Americas. However, the underlying mechanisms through which ZIKV rapidly and explosively spread from Asia to the Americas are unclear. Non-structural protein 1 (NS1) facilitates flavivirus acquisition by mosquitoes from an infected mammalian host and subsequently enhances viral prevalence in mosquitoes. Here we show that NS1 antigenaemia determines ZIKV infectivity in its mosquito vector Aedes aegypti, which acquires ZIKV via a blood meal. Clinical isolates from the most recent outbreak in the Americas were much more infectious in mosquitoes than the FSS13025 strain, which was isolated in Cambodia in 2010. Further analyses showed that these epidemic strains have higher NS1 antigenaemia than the FSS13025 strain because of an alanine-to-valine amino acid substitution at residue 188 in NS1. ZIKV infectivity was enhanced by this amino acid substitution in the ZIKV FSS13025 strain in mosquitoes that acquired ZIKV from a viraemic C57BL/6 mouse deficient in type I and II interferon (IFN) receptors (AG6 mouse). Our results reveal that ZIKV evolved to acquire a spontaneous mutation in its NS1 protein, resulting in increased NS1 antigenaemia. Enhancement of NS1 antigenaemia in infected hosts promotes ZIKV infectivity and prevalence in mosquitoes, which could have facilitated transmission during recent ZIKV epidemics.

| S-EPMC5885636 | biostudies-literature

CD47

Project description:Macaca mulatta CD47, CD47 molecule [Source:VGNC Symbol;Acc:VGNC:70767], is expressed in 3 baseline experiment(s);

| ENSMMUG00000008791 | ExpressionAtlas

CD47

Project description:Equus caballus CD47, CD47 molecule [Source:VGNC Symbol;Acc:VGNC:57080], is expressed in 1 baseline experiment(s);

| ENSECAG00000018995 | ExpressionAtlas

CD47

Project description:Homo sapiens CD47, CD47 molecule [Source:HGNC Symbol;Acc:HGNC:1682], is expressed in 60 baseline experiment(s);

| ENSG00000196776 | ExpressionAtlas