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Effect of roots subjected to 300 mg/l PS-COOH treatments and to control conditions for RNA-Seq in cotton

ABSTRACT: Effect of roots subjected to 300 mg/l PS-COOH treatments and to control conditions for RNA-Seq in cotton

PROVIDER: PRJNA1001952 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25548170_1.fastq.gz	Fastqsanger.gz
	SRR25548170_2.fastq.gz	Fastqsanger.gz
	SRR25548171_1.fastq.gz	Fastqsanger.gz
	SRR25548171_2.fastq.gz	Fastqsanger.gz
	SRR25548172_1.fastq.gz	Fastqsanger.gz

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Effect of roots subjected to 300 mg/l PS-COOH treatments and to control conditions for RNA-Seq in cotton

Project description:Roots from 10 independent plants subjected to 300 mg/l PS-COOH treatments and to control conditions were pooled separately to form single biological replicates for RNA-seq analysis. Transcriptomics analyses showed that antioxidant activity-related genes were significantly upregulated after PS-COOH treatments, indicating that oxidative stress was induced in cotton.

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Transcription profiling of total of rat liver samples treated with 300 mg/kg/day myclobutanil, 300 mg/kg/day propiconazole, and 175 mg/kg/day triadimefon

Project description:The modes of triazole reproductive toxicity have been characterized by an observed increased in serum testosterone and reduced insemination and fertility indices. The key events involved in the disruption in testosterone homeostasis and reduced fertility remain unclear. Gene expression analysis was conducted on liver from Sprague Dawley rats dosed with myclobutanil (300 mg/kg/day), propiconazole (300 mg/kg/day), or triadimefon (175 mg/kg/day) for 72 hours. Pathway-based analysis highlighted key biological processes affected by all three triazoles in the liver including fatty acid catabolism, steroid metabolism, and xenobiotic metabolism. Within the pathways identified in the liver, specific genes involved in phase I-III metabolism and fatty acid metabolism were affected by all three triazoles. These modulated genes are part of a network of lipid and testosterone homeostasis pathways regulated by the constitutive androstane (CAR) and pregnane X (PXR) receptors. Gene expression profiles from this study indicate triazoles activate CAR and PXR; increase fatty acid catabolism and steroid metabolism in the liver; constituting a plausible series of key events contributing to the observed disruption in testosterone homeostasis. Experiment Overall Design: A total of 12 liver samples were analyzed. Three biological replicates each for the controls, 300 mg/kg/day myclobutanil, 300 mg/kg/day propiconazole, and 175 mg/kg/day triadimefon.

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Methanococcus voltae PS strain:PS

Project description:Methanococcus voltae PS transcriptome - H37-1-PS

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