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Evaluating the toxicity of two endocrine disrupting chemicals, BPA and TBBPA using a larval zebrafish transcriptomic approach

ABSTRACT: Evaluating the toxicity of two endocrine disrupting chemicals, BPA and TBBPA using a larval zebrafish transcriptomic approach

PROVIDER: PRJNA1002312 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25528725_1.fastq.gz	Fastqsanger.gz
	SRR25528725_2.fastq.gz	Fastqsanger.gz
	SRR25528726_1.fastq.gz	Fastqsanger.gz
	SRR25528726_2.fastq.gz	Fastqsanger.gz
	SRR25528727_1.fastq.gz	Fastqsanger.gz

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Evaluating the toxicity of two endocrine disrupting chemicals, BPA and TBBPA using a larval zebrafish transcriptomic approach

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In vitro transcriptomic analyses reveal pathway perturbations, estrogenic activities, and potencies of data-poor BPA alternative chemicals

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Exposure to environmental chemicals alters human mammary gland morphogenesis and proteome

Project description:Environmental chemicals such as bisphenol A (BPA) are thought to contribute to carcinogenesis through their endocrine-disrupting effects. However BPA replacement chemicals are structurally similar, and little data exist describing their effects on the human body and environment. We established non-malignant human mammary organoid cultures to investigate the effects of BPA replacement chemicals on organoid morphology and protein abundance. At low-nanomolar doses, replacement chemicals, in particular BPS, induced branching and disrupted the organized mammary organoid architecture. BPS exposure likely alters mammary cell-type proportions and induces branching of predominately myoepithelial cells. Treatment with different replacement chemicals resulted in distinct proteomic changes. BPS exposure induced Cdc42-interacting protein 4 (CIP4), a protein known to support invadopodia formation and mesenchymal phenotypes. Our study provides evidence that replacement bisphenols have pro-tumorigenic effects on mammary morphology and the proteome, highlighting the necessity of comprehensive studies to evaluate the potential harm of replacement chemicals.

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