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Effects of the onecut family on herpes simplex virus 1 and host chromatin accessibility in Neuro-2a cells

ABSTRACT: Effects of the onecut family on herpes simplex virus 1 and host chromatin accessibility in Neuro-2a cells

PROVIDER: PRJNA1002997 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25557649_1.fastq.gz	Fastqsanger.gz
	SRR25557649_2.fastq.gz	Fastqsanger.gz
	SRR25557650_1.fastq.gz	Fastqsanger.gz
	SRR25557650_2.fastq.gz	Fastqsanger.gz
	SRR25557651_1.fastq.gz	Fastqsanger.gz

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Effects of the onecut family on herpes simplex virus 1 and host chromatin accessibility in Neuro-2a cells

Project description:This is a part of the study that shows that a host gene,ONECUT2( OC2), promotes herpes simplex virus 1 (HSV-1) genome accessibility. These ATAC analyses are for viral and host genome accessibility in Neuro-2a cells. Neuro-2a cells were transfected with pOC2△HOX2 and pcDNA plasmids for 42 hours then infected with herpes simple virus1 for 2 hours.

2023-09-18 | GSE240221 | GEO

RNA-seq identification of herpes simplex virus type 1 transcription in Neuro-2a cells overexpressing ONECUT2

Project description:This is a part of the study that shows that a host gene,ONECUT2 (OC2), promote herpes simplex virus 1 (HSV-1) transcription. These RNA-seq analyses viral genes transcription in Neuro-2a cells. Neuro-2a cells were transfected with pOC2△HOX2 and pcDNA plasmids for 42 hours then infected with herpes simple virus1 for 5 hours.

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Effects of the FOXF1 on herpes simplex virus 1 and host chromatin accessibility in Neuro-2a cells

Project description:This is a part of the study that shows that a host gene,FOXF1, promote herpes simplex virus 1 (HSV-1) genome accessibility. These ATAC analyses viral and host genome accessibility in Neuro-2a cells. Neuro-2a cells were transfected with pFOXF1 and pcDNA plasmids for 42 hours then infected with herpes simple virus1 for 3 hours.

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Identification of herpes simplex virus 1 and host targets of miR-138 by comparative PAR-CLIP in Neuro-2a and 293T cells

Project description:This is a part of the study that shows that a host microRNA, miR-138, represses herpes simplex virus 1 (HSV-1) gene expression through both viral and host targets. These PAR-CLIP analyses identified viral and host targets of miR-138 in Neuro-2a (mouse neuroblastoma) and 293T (human embryonic kidney) cells. We constructed two cell lines derived from Neuro-2a cells, one overexpressing miR-138 (N2A138) and one antagonizing miR-138 (N2Aanti138). We also constructed two cell lines derived from 293T cells, one overexpressing miR-138 (293T138) and one control cells (293Tcontrol). Uninfected N2A138 and N2Aanti138 were compared by PAR-CLIP for host targets in Neuro-2A cells. 293T138 and 293Tcontrol cells infected for 4 and 8 hours were compared by PAR-CLIP for HSV-1 targets in 293T cells. 293T138 and 293Tcontrol cells infected for 4 hours were also compared for host targets in 293T cells.

2020-04-27 | GSE127503 | GEO

RNA-seq identification of herpes simplex virus type 1 transcription in Neuro-2a cells overexpressing ONECUT2

Project description:RNA-seq identification of herpes simplex virus type 1 transcription in Neuro-2a cells overexpressing ONECUT2

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Identification of the binding DNA of FOXF1 by ChIP in Neuro-2a cells

Project description:Neuro-2a cells were transfected with pFOXF1 for 42 hours then infected with herpes simple virus1 for 5 hours. Samples infected for 5 hours were sequenced by ChIP for binding DNAs of the FOXF1 in Neuro-2a cells.

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Effects of the FOXF1 on herpes simplex virus 1 and host chromatin accessibility in Neuro-2a cells

Project description:Effects of the FOXF1 on herpes simplex virus 1 and host chromatin accessibility in Neuro-2a cells

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Project description:RNA-seq identification of herpes simplex virus type 1 transcription in Neuro-2a cells overexpressing FOXK1 and FOXF1

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Effects of host miR-138 on herpes simplex virus 1 gene expression in Neuro-2a cells and their dependence of the binding sites on ICP0 mRNA

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