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ABSTRACT: Transcriptome sequencing of pear

PROVIDER: PRJNA1004416 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25642100_1.fastq.gz	Fastqsanger.gz
	SRR25642100_2.fastq.gz	Fastqsanger.gz
	SRR25642095_1.fastq.gz	Fastqsanger.gz
	SRR25642095_2.fastq.gz	Fastqsanger.gz
	SRR25642096_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis reveals the mechanism of watercore occurrence and its accelerating fruit decay

Project description:purpose：The purpose of this study was to compare the transcriptome data of the diseased and the non diseased pear to verify the changes of different physiological indexes of the diseased pear Methods：By sequencing the transcriptome of the treatment group and the control group, the differences of genes related to glycoalcohol metabolism and cell wall physiological pathway metabolism between the two groups were compared. Verification by fluorescence quantitative PCR

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Comparative Transcriptome Analysis Reveals Insight into Exocarp Russeting Pear Variety

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Comparative Transcriptome Analysis Reveals New Insight into Russeting of the Semi-Russeted ‘Cuiguan’ Pear Variety

Project description:We carried out the transcriptome analysis to identify the key genes involved in pear fruit semi-russet formation, by comparing the CK (russet) and bagging treated (green) ‘Cuiguan’ pear fruit skin 115 DAFB.

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Comparative Transcriptome Analysis Reveals New Insight into Russeting of the Semi-Russeted ‘Cuiguan’ Pear Variety

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Comparative Transcriptome Analysis of callery pear (Pyrus calleryana Decne) seeds at three different stages of cold stratification

Project description:We carried out the transcriptome analysis to explore expression profiles differences and identify the key genes involved in pear seed dormancy release, by comparing callery pear (Pyrus calleryana Decne) seeds at three different stages of cold stratification

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Comparative Methylome Analysis Reveals New Insight into Russeting of the Semi-Russeted ‘Cuiguan’ Pear Variety [Bisulfite-seq]

Project description:We carried out genomewide DNA methylation analysis by whole genome bisulfite sequencing to identify candidate genes involved in pear fruit semi-russet formation, by comparing the CK (russet) and bagging treated (green) ‘Cuiguan’ pear fruit skin 115 DAFB.

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Transcriptomic and metabolomic analysis provides insights into anthocyanin and procyanidin accumulation in pear

Project description:Background: Pear is one of the most important fruit crops worldwide. Anthocyanins and procyanidins (PAs) are important secondary metabolites that affect the appearance and nutritive quality of pear. However, few studies have focused on the molecular mechanism underlying anthocyanin and PA accumulation in pear. Results: We conducted metabolome and transcriptome analyses to identify candidate genes involved in anthocyanin and PA accumulation in young fruits of the pear cultivar ‘Clapp Favorite’ (CF) and its red mutation cultivar ‘Red Clapp Favorite’ (RCF). Gene–metabolite correlation analyses revealed a ‘core set’ of 20 genes that were strongly correlated with 10 anthocyanin and seven PA metabolites. Of these, PcGSTF12 was confirmed to be involved in anthocyanin and PA accumulation by complementation of the tt19-7 Arabidopsis mutant. Interestingly, PcGSTF12 was found to be responsible for the accumulation of procyanidin A3, but not petunidin 3, 5-diglucoside, opposite to the function of AtGSTs in Arabidopsis. Transformation with PcGSTF12 greatly promoted or repressed genes involved in anthocyanin and PA biosynthesis, regulation, and transport. Electrophoretic mobility shift and luciferase reporter assays confirmed positive regulation of PcGSTF12 by PcMYB114. Conclusion: These findings identify a core set of genes for anthocyanin and PA accumulation in pear. Of these, PcGSTF12 was confirmed to be involved in anthocyanin and PA accumulation. Our results also identified an important anthocyanin and PA regulation node comprising two core genes, PcGSTF12 and PcMYB114. These results provide novel insights into anthocyanin and PA accumulation in pear and represent a valuable data set to guide future functional studies and pear breeding.

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Project description:Comparative analyze at the transcriptomic level 1) of Venturia pyrina pear host resistance via the major apple resistance gene Rvi6, in Rvi6 overexpressing transgenic pear versus ‘conference’ susceptible variety; 2) of Venturia inaequalis pear nonhost resistance, in ‘Conference’ variety, 24 and 72 hours post inoculation.

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Next Generation Sequencing Facilitates Quantitative Analysis of miRNA

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