Project description:We sequenced mRNA from the different development stage of G. biloba embryo using the Illumina HiSeq 4000 platform to generate the transcriptome dynamics to explore ginkgo embryo development mechanism of post-maturation and lay the foundation for revealing the molecular mechanisms of seed dormancy and germination of G. biloba seed.
Project description:We sequenced mRNA from Ginkgo biloba leaves grown at different developmental stages using the Illumina HiSeq4000 platform to generate the transcriptome dynamics that may serve as a gene expression profile blueprint for different response patterns during autumn leaf senescence and coloration. These results contribute to the elucidation of the molecular mechanisms involved in the leaf coloration and senescence in G. biloba as well as to the identification of candidate genes involved in this process.
Project description:To explore the overall long noncoding RNA (lncRNA) involved in major developmental stages of Ginkgo biloba leaves , we deeply sequenced samples of leaves from different developmental stages (from April to October) using strand-specific RNA sequencing (ssRNA-seq) menthod. We obtained 27.44 Gb raw data and identified 1323 novel lncRNAs. We also categorized the novel lncRNAs as intergenic, intronic, antisense and sense based on their location on theGinkgo biloba genome. Furthermore, lncRNAs targeted protein-coding genes were predicted and functional annotated. In addition, we constructed a network of interactions between ncRNAs (miRNAs, lncRNA) and mRNAs. Our results suggest that the identified novel lncRNAs are important in modulating development process of Ginkgo biloba, and provide a rich resource for further research on the function of these novel lncRNAs.
Project description:We conducted RNA-seq from the Ginkgo leaves after UV-B treatment,and constructed the molecular regulatory network of flavonoids synthesis under UV-B radiation in G. biloba.
