Project description:We performed that comprehensive identification of genes responsible for stress tolerance by analyzing the whole-genome expression profiles of poplar (Populus alba × P. glandulosa) leaves exposed to drought and salt stresses. Examination at the molecular level how this tree species responds to drought and salt stresses by regulating the expression of genes involved in signal transduction, transcriptional regulation, and stress responses.
Project description:Transcriptome analysis was performed on the sixth leaves of Populus alba × P. glandulosa. The twelve samples were: four PagSUPa overexpression (OE) line 2 samples 2-1-L6, 2-2-L6, 2-3-L6 and 2-4-L6, four PagSUPa OE line 7 samples 7-1-L6, 7-2-L6, 7-3-L6 and 7-4-L6, four nontransgenic control (CK) samples CK-1-L6, CK-2-L6, CK-3-L6, CK-4-L6 . And the differential genes between PagSUPa OE and CK groups were identified and compared, which helps to reveal the molecular mechanism of PagSUPa directly suppresses the expression of phragmoplast orienting and positioning genes PagPOK1 and PagPOK2, and impairs cytokinesis and cell wall organization.
Project description:We performed that comprehensive identification of genes responsible for stress tolerance by analyzing the whole-genome expression profiles of poplar (Populus alba M-CM-^W P. glandulosa) leaves exposed to drought and salt stresses. Examination at the molecular level how this tree species responds to drought and salt stresses by regulating the expression of genes involved in signal transduction, transcriptional regulation, and stress responses. Genome-wide analysis was conducted in poplar leaves exposed to drought and salt stresses.The plants were acclimated in soil and grown for 6 weeks in controlled conditions in a growth room (16 h light; light intensity, 150 M-NM-<mol m-2sec-1; 24M-BM-0C). Plants with a height of about 15 cm were separately exposed to either drought or salt stress. Up- and down-regulated genes were identified, and their putative functions are discussed.
