Project description:The mammalian body plan is established during symmetry breaking and gastrulation. In ungulates and primates, these events occur within a flat embryonic disc and coincide with the developmental window most susceptible to pregnancy loss. Using the EvercodeTM Cell Fixation kit v2 (ECF2101, Parse Biosciences), we have generated a single-cell RNA sequencing atlas of in vivo sheep embryos that defines the timing and molecular programs underlying lineage segregation, as well as the inter-lineage signalling mediating anterior visceral hypoblast specification (AVH) and primitive streak formation.
Project description:Zygotic genome activation (ZGA) is essential for early embryo development. Here, we reported that ZGA is initiated at 16-cell stage in sheep, as extensive alterations in gene expression and DNA methylation patterns, along with elevated levels of RNA polymerase II and developmental arrest was found at the 16-cell embryos. To uncover the sophisticated RNA metabolism during ZGA, we conducted weighted gene co-expression network analysis and identified 1957 critical maternal genes. Using dapars, we found 1058 and 933 lengthened alternative polyadenylation (APA) events, and genes exhibiting shorten APA were highly expressed in both sheep and mice ZGA-stage embryos. Using rMATs, we reported 2675 and 1963 genes showed exon skipping during ZGA in sheep and mice. These genes are related to RNA binding, translation, gamete generation, and reproduction.
Project description:The uploaded proteome data were obtained from 336 samples of perinatal Bama miniature pigs, covering multiple time points and brain regions. Analyzed with transcriptome data, they revealed the spatiotemporal organization of perinatal molecular profiles and gene-protein expression concordance. These data helped confirm perinatal Bama miniature pigs as an optimal large-animal model for human perinatal neurodevelopment (closer to humans than mice). They also identified a pan-regional developmental inflection point (E94-E104) with dramatic changes in proteins related to synaptogenesis and gliogenesis, supported analyses of regional developmental heterogeneity, and facilitated studying brain disorder risk gene transitions, serving as a key part of the multi-omics atlas for decoding neurodevelopmental pathogenesis.
Project description:To explore functional lncRNAs during sheep muscle growth, we systematically investigated lncRNAs using strand-specific Ribo-Zero RNA Sequencing at three key developmental stages of Hu sheep (110-day fetus, 5-day-old lamb and 2-year-old adult)
