Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA100739 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:circadian cycle-The impact of some genes mutations implicated in the amidon metabolism
| PRJNA100737 | ENA
Project description:af30_starch_circadian_rythm - double mutant sbe - The scientific aim is to study the impact of some genes mutations implicated in the amidon metabolism on the expression profils of other genes and in particular those of the carbonated metabolism. Those analyses will be done from RNAs (total or messenger) extracted from leaves at various time of the day and the night - Biological question : what is the impact of the absence of both branching enzymes II on the expression of the genes involved in carbon metabolism in the Arabidopsis leaves? Keywords: gene knock out
2007-06-20 | GSE7976 | GEO
Project description:MethylC-Seq of idm2 single mutant and idm1idm2 double mutant
| PRJNA213861 | ENA
Project description:RNA-seq of Arabidopsis thaliana Col-0, rsl2 rsl4 double mutant and bdr6 bdr7 double mutant. (PRJCA042698)
| PRJDB38886 | ENA
Project description:Arabidopsis stn7-stn8 double mutant vs WT
| PRJNA104761 | ENA
Project description:Mitochondria play important roles in the plant stress responses and the detoxification of the reactive oxygen species generated in the electron-transport chain. Expression of genes encoding stress-related proteins such as the mitochondrial small heat shock proteins (M-sHSP) is upregulated in response to different abiotic stresses. In Arabidopsis thaliana, three M-sHSPs paralogous genes were identified, although their function under physiological conditions remains elusive. Here, we analyzed the phenotype, proteomic and metabolic profiles of the loss-of-function mutants of M-sHSPs (single, double and triple mutants) during normal plant growth. The triple mutant showed the most prominent altered phenotype at vegetative and reproductive stages without any externally applied stress. They displayed chlorotic leaves, growth arrest and low seed production. Concomitantly, they exhibited increased levels of sugars, free amino acids such as proline, citric and ascorbic acid, among other metabolites. Single and double mutants displayed intermediate phenotype suggesting a redundant function of these proteins. All single, double and triple mutants showed alteration of proteins involved in photosynthesis, mitochondrial metabolism and antioxidant defense compared to the wild-type plants. Overall, depletion of M-sHSPs causes severe impact in fundamental metabolic processes, localized in different cell compartments, leading to alterations in the correct plant growth and development.
2021-01-28 | PXD019603 | Pride
Project description:Root transcriptome of the tsn1 tsn2 double mutant
| PRJNA115649 | ENA
Project description:Transcriptomic analysis of the voz1 voz2 double mutant
| PRJNA132133 | ENA
Project description:ra10-01_laccases; laccase mutations. We demonstrated that laccases are involved in lignin polymerisation. Mutants have already been tested on microarrays and there is few differences compared to wild-type. The laccase mutation seems surgical. We possess a new double mutant, called snips, with a semi-dwarf phenotype, and we want to determine its profile.
2011-07-02 | GSE22907 | GEO
Project description:Defects in RNA maturation and RNA decay factors may generate substrates for the RNA interference machinery. This phenomenon was observed in plants where mutations in some RNA-related factors lead to the production of RNA-quality control small interfering RNAs and several mutants show enhanced silencing of reporter transgenes. To assess the potential of RNAi activation on endogenous transcripts, we sequenced small RNAs from a set of Arabidopsis thaliana mutants with defects in various RNA metabolism pathways. We observed a global production of siRNAs caused by inefficient pre-mRNA cleavage and polyadenylation leading to read-through transcription into downstream antisense genes. In addition, in the lsm1a lsm1b double mutant, we identified NIA1, SMXL5, and several miRNA-targeted mRNAs as producing siRNAs, a group of transcripts suggested being especially sensitive to deficiencies in RNA metabolism. However, in most cases, RNA metabolism perturbations do not lead to the widespread production of siRNA derived from mRNA molecules. This observation is contrary to multiple studies based on reporter transgenes and suggests that only a very high accumulation of defective mRNA species caused by specific mutations or substantial RNA processing defects trigger RNAi pathways. Founded by National Science Centre Grant, Poland (UMO-2013/08/M/NZ1/00931, UMO-2018/29/B/NZ3/01980).
2022-02-02 | GSE169171 | GEO