Project description:Transcriptional profiling of hypocotyl comparing wild type with shr mutant. We used Affymetrix ATH1 microarrays to determine the effect of GRAS transcription factor SHORT-ROOT on growth and development of Arabidopsis shoot system (hypocotyl) by global transcriptome analysis and to identify the key players in the regulatory pathway.
Project description:Here, in a time-course phosphoproteome analysis on Arabidopsis seedlings exposed to control or high ambient temperature, we observed reduced levels of diverse proteins over time, which could be in part due to transcription, translation and/or degradation. In addition, we observed differential phosphorylation of the LRR F-box protein SLOMO MOTION (SLOMO) at two serine residues. We demonstrated that SLOMO is a negative regulator of hypocotyl growth, and protein-protein interaction studies revealed possible interactors of SLOMO.
