Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy

ABSTRACT: Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy

PROVIDER: PRJNA1013387 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25918002_1.fastq.gz	Fastqsanger.gz
	SRR25918002_2.fastq.gz	Fastqsanger.gz
	SRR25918003_1.fastq.gz	Fastqsanger.gz
	SRR25918003_2.fastq.gz	Fastqsanger.gz
	SRR25918004_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 26

Similar Datasets

Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy

Project description:Loss of pancreatic beta cells is the central feature of all forms of diabetes. Current therapies fail to halt the declined beta cell mass. Thus, strategies to preserve beta cells are imperatively needed. In this study, we identified paired box 6 (PAX6) as a critical regulator of beta cell survival. Under diabetic conditions, the human beta cell line EndoC-bH1, db/db mouse and human islets displayed dampened insulin and incretin signalings and reduced beta cell survival, which were alleviated by PAX6 overexpression. Adeno-associated virus (AAV)-mediated PAX6 overexpression in beta cells of streptozotocin-induced diabetic mice and db/db mice led to a sustained maintenance of glucose homeostasis. AAV-PAX6 transduction in human islets reduced islet graft loss and improved glycemic control after transplantation into immunodeficient diabetic mice. Our study highlights a previously unappreciated role for PAX6 in beta cell survival and raises the possibility that ex vivo PAX6 gene transfer into islets prior to transplantation might enhance islet graft function and transplantation outcome.

2023-10-13 | GSE242338 | GEO

Paired box 6 gene delivery preserves beta cells and improves islet transplantation efficacy.

Project description:Loss of pancreatic beta cells is the central feature of all forms of diabetes. Current therapies fail to halt the declined beta cell mass. Thus, strategies to preserve beta cells are imperatively needed. In this study, we identified paired box 6 (PAX6) as a critical regulator of beta cell survival. Under diabetic conditions, the human beta cell line EndoC-βH1, db/db mouse and human islets displayed dampened insulin and incretin signalings and reduced beta cell survival, which were alleviated by PAX6 overexpression. Adeno-associated virus (AAV)-mediated PAX6 overexpression in beta cells of streptozotocin-induced diabetic mice and db/db mice led to a sustained maintenance of glucose homeostasis. AAV-PAX6 transduction in human islets reduced islet graft loss and improved glycemic control after transplantation into immunodeficient diabetic mice. Our study highlights a previously unappreciated role for PAX6 in beta cell survival and raises the possibility that ex vivo PAX6 gene transfer into islets prior to transplantation might enhance islet graft function and transplantation outcome.

| S-EPMC10701606 | biostudies-literature

Antioxidant program of islet-resident macrophages preserves β cell physiology

Project description:Antioxidant program of islet-resident macrophages preserves β cell physiology

| PRJNA1035521 | ENA

Antioxidant program of islet-resident macrophages preserves β cell physiology

Project description:Antioxidant program of islet-resident macrophages preserves β cell physiology

| PRJNA1029611 | ENA

Antioxidant program of islet-resident macrophages preserves β cell physiology

Project description:Antioxidant program of islet-resident macrophages preserves β cell physiology

| PRJNA1035525 | ENA

Antioxidant program of islet-resident macrophages preserves β cell physiology [dataset 4]

Project description:Antioxidant program of islet-resident macrophages preserves β cell physiology [dataset 4]

| PRJNA1322428 | ENA

Antioxidant program of islet-resident macrophages preserves β cell physiology [dataset 3]

Project description:Antioxidant program of islet-resident macrophages preserves β cell physiology [dataset 3]

| PRJNA1322423 | ENA

Antioxidant program of islet-resident macrophages preserves β cell physiology

Project description:Macrophages residing within mouse pancreatic islets display an activated phenotype at steady state, yet the molecular signatures underlying their polarization remain unclear. To investigate islet macrophage heterogeneity under physiological conditions, we isolated islet-resident immune cells from chow diet-fed adult male mice and performed single-cell RNA sequencing.

2025-11-14 | GSE245717 | GEO

Antioxidant program of islet-resident macrophages preserves β cell physiology

Project description:Macrophages residing inside mouse pancreatic islets have an activated phenotype at steady-state. To explore the molecular pathways involved in their polarization, we compared the transcriptomic signatures of islet-resident macrophages with autologous epididymal adipose tissue (EpiWAT)-resident macrophages. Our data confirmed the pro-inflammatory profile of islet marophages and revealed also strong anti-oxidant capacities compared to EpiWATcells.

2025-11-17 | GSE246999 | GEO

The Atlas of Immune Infiltrates in Islet Cell Allo-transplantation

Project description:Islet transplantation to treat the late stage of T1DM patients has made some inspiring success recently in clinical trials. However, most of patients underwent a decline of islet cell graft in one to three years due to chronic immune rejection. Although the mechanisms of immune cells including macrophages, dendritic cells (DCs), neutrophils, natural killer cells (NKs), B cells, T cells that mediate immune rejection have been investigated, the overall characteristics of immune infiltrates in islet allografts remain unclear. Single-cell RNA sequencing (scRNA-seq) has provided us with new opportunities to study the molecular characteristics of the immune microenvironment in islet transplants. In the present study, we used scRNA-seq to comprehensively analyze the immune heterogeneity in islet graft, and compared the variances regarding to transcriptomal profiling and immune atlas between syngeneic islet transplantation and allograft.

2022-05-25 | GSE198865 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data