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Shewanella putrefaciens

ABSTRACT: Shewanella putrefaciens Genome sequencing and assembly

PROVIDER: PRJNA1015196 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR26037686_1.fastq.gz	Fastqsanger.gz
	SRR26037686_2.fastq.gz	Fastqsanger.gz
	SRR26037687_1.fastq.gz	Fastqsanger.gz
	SRR26037687_2.fastq.gz	Fastqsanger.gz
	SRR26037688_1.fastq.gz	Fastqsanger.gz

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Project description:Purpose : Identification of the regulons directly and indirectly affected by the main regulators of flagellation in Shewanella putrefaciens CN-32 Methods : mRNA profiles were generated for Shewanella putrefaciens CN-32 samples by deep sequencing. The removal of ribosomal RNA was performed using the Ribo-Zero Bacteria Kit (Illumina) and cDNA libraries were generated with the ScriptSeq v2 Kit (Illumina) . The samples were sequenced in single end mode on an Illumina HiSeq 2500 device and mRNA reads were trimmed using the tool ‘cutadapt’ (version 3.5) with default settings and mapped to the NC_009438.1 (Shewanella putrefaciens CN-32) reference genome from NCBI using ‘bowtie2’ (version 2.3.5.1) with default settings for single-end sequencing.

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