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Metabolic adaptation pilots the differentiation of human hematopoietic cells (scATAC-Seq)

ABSTRACT: Metabolic adaptation pilots the differentiation of human hematopoietic cells (scATAC-Seq)

PROVIDER: PRJNA1015713 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26046012_1.fastq.gz	Fastqsanger.gz
	SRR26046012_2.fastq.gz	Fastqsanger.gz
	SRR26046013_1.fastq.gz	Fastqsanger.gz
	SRR26046013_2.fastq.gz	Fastqsanger.gz
	SRR26046014_1.fastq.gz	Fastqsanger.gz

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Metabolic adaptation pilots the differentiation of human hematopoietic cells

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Project description:Metabolic cues are crucial for regulating hematopoietic stem and progenitor cells (HSPCs). However, the metabolic profile of human HSPCs remains poorly understood due to the limited number of cells and the scarcity of bone marrow samples. Here, we present the integrated metabolome, lipidome and transcriptome of human adult HSPCs (lineage-, CD34+, CD38-) upon differentiation, aging and acute myeloid leukemia (AML). The combination of low-input targeted metabolomics with our newly optimized low-input untargeted lipidomics workflow allows us to detect up to 219 metabolites and lipids from a starting material of 3,000 and 5,000 HSPCs, respectively. Among other findings, we observe elevated levels of the essential nutrient choline in HSPCs compared to downstream progenitors, which decline upon aging and further decrease in AML. Functionally, we show that choline supplementation fuels lipid production in HSPCs and enhances stemness. Overall, our study provides a comprehensive resource identifying metabolic changes that can be utilized to promote and enhance human stem cell function.

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Project description:We report here the analysis of young and aged hematopoietic stem and progenitor cells (HSPCs) by transcriptional profiling (bulk RNA-Seq, CITE-Seq) and chromatin profiling (ATAC-Seq, scATAC-Seq). We use here a Clca3a1 as a surface marker to further fractionate HSPCs. HSPCs were sorted by flow cytometry. LT-HSCs were fractionated into a Clca3a1 high or low population using a monoclonal antibody (10.1.1) purchased from the Developmental Studies Hybridoma Bank - DSHB.

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