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ABSTRACT: Aglais urticae RNA sequence

PROVIDER: PRJNA1017429 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR26089670_1.fastq.gz	Fastqsanger.gz
	SRR26089670_2.fastq.gz	Fastqsanger.gz
	SRR26089671_1.fastq.gz	Fastqsanger.gz
	SRR26089671_2.fastq.gz	Fastqsanger.gz
	SRR26089672_1.fastq.gz	Fastqsanger.gz

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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of differentially expressed genes (DEG) between the treated samples of mites.The goal of the study is to elucidate the invasion mechanism that results in the continuous expansion of Tetranychus urticae that has occurred in China. Methods:Transcriptome profiling by RNA sequencing is becoming an attractive method as it facilitates rapid generation, identification and quantification of large number of transcripts, even in the species where no prior genome sequence information is available. Results:the expression of 10 P450(cytochrome P450 monooxygenases), 6 GST(glutathione S-transferases), 13 CCE(carboxy/cholinesterases) and other novel candidate genes was higher (>2-fold) in T. urticae than in T. cinnabarinus as detected by using the digital gene expression (DGE) method.

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Project description:While performing mitochondrial isolations and recently developed tRNA-seq methods (AlkB treatment and YAMAT-Seq) in plant tissue, we inadvertently sequenced the mitochondrial tRNAs from a common plant pest, the acariform mite Tetranychus urticae, to a high enough coverage to detect all previously annotated T. urticae tRNA regions. The results not only confirm expression, CCA-tailing and post-transcriptional base modification of these highly divergent tRNAs, but also revealed paired sense and antisense expression of multiple T. urticae mitochondrial tRNAs.

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Project description:Gene expression differences between MR-VL_FBO, a T. urticae strain highly resistant to fenbutatin oxide, and KOP8, a T. urticae strain susceptible to fenbutatin oxide, were determined.

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