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ABSTRACT: Transcriptional sequencing of lip in Cymbidium floribundum

PROVIDER: PRJNA1017809 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26070015_1.fastq.gz	Fastqsanger.gz
	SRR26070015_2.fastq.gz	Fastqsanger.gz
	SRR26070016_1.fastq.gz	Fastqsanger.gz
	SRR26070016_2.fastq.gz	Fastqsanger.gz
	SRR26070017_1.fastq.gz	Fastqsanger.gz

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Project description:summary (1)Objective: To investigate the antileukemic role of Lip-1 in K562 leukemia cells. (2)Methods: We performed CCK-8, flow cytometry, microscopy, western blotting assay, next generation sequencing, PCR assays to evaluate the effect of Lip-1 in K562 leukemia cells. (3)Results: Lip-1 inhibited K562 cell proliferation in a dose- and time-dependent manner. RNA sequencing revealed several pathways that were affected by Lip-1, such as the G1/S transition of the mitotic cell cycle and extrinsic or intrinsic apoptotic signaling pathways. The results of flow cytometry indicated that Lip-1 arrests the cell cycle. K562 cells were characterized by swelling and plasma membrane rupture. The expression of the hallmark of pyroptosis, the cleaved N-terminal GSDME, increased. Endoplasmic reticulum stress and autophagy were involved in Lip-1-induced cell death. (4)Conclusion：Lip-1 induces cell cycle arrest, apoptosis, and secondary pyroptosis in K562 leukemia cells, which provides new hope for the treatment of leukemia.

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