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ABSTRACT: Microbial sample from parageobacillus thermoglucosidasius

PROVIDER: PRJNA1018439 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26105773_1.fastq.gz	Fastqsanger.gz
	SRR26105773_2.fastq.gz	Fastqsanger.gz
	SRR26105774_1.fastq.gz	Fastqsanger.gz
	SRR26105774_2.fastq.gz	Fastqsanger.gz

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Analysis of proteome dynamics in early-stage sporulation-inhibited variants of Parageobacillus thermoglucosidasius

Project description:Parageobacillus thermoglucosidasius is a thermophilic Gram-positive that is currently on the rise as a potential platform for sustainable chemical production. One major issue with P. thermoglucosidasius as a host is its natural ability to sporulate in response to nutrient starvation, as endospore formation is generally undesired in industrial fermentations. It is therefore of interest to instead employ a sporulation-supressed variant of P. thermoglucosidasius. However, as a non-model species, the complex regulatory system that governs sporulation in P. thermoglucosidasius is currently poorly characterised. This study presents a characterisation and proteomic analysis of the P. thermoglucosidasius wildtype strain alongside four early-stage sporulation-inhibited variants. The genes targeted for sporulation-inhibition were Spo0A, Spo0B, Spo0F, and SigF, based on their regulatory roles in the sporulation pathway of Bacillus subtilis. Proteomics data was collected from four different growth stages and the resulting expression profiles were compared. This led to the identification of a co-expression module consisting of approximately 200 genes, which through enrichment was confirmed to be closely linked to the P. thermoglucosidasius sporulation pathway. The results of the analysis have been made publicly available.

2025-12-22 | PXD069977 | Pride

Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasius

Project description:Given sustainability and scalability concerns of using sugar feedstocks for microbial bioproduction of bulk chemicals, widening the feedstock range for microbial cell factories is of high interest. Methanol is a one-carbon alcohol that stands out as an alternative feedstock for the bioproduction of chemicals, as it is electron-rich, water-miscible and can be produced from several renewable resources. Bioconversion of methanol into products under thermophilic conditions (>50 °C) could be highly advantageous for industrial biotechnology. Although progress is being made with natural, thermophilic methylotrophic microorganisms, they are not yet optimal for bioproduction and establishing alternative thermophilic methylotrophic bioproduction platforms can widen possibilities. Hence, we set out to implement methanol assimilation in the emerging thermophilic model organism Parageobacillus thermoglucosidasius. We engineered P. thermoglucosidasius to be strictly dependent for its growth on methanol assimilation via the core of the highly efficient ribulose monophosphate (RuMP) cycle, while co-assimilating ribose. Surprisingly, this did not require heterologous expression of RuMP enzymes. Instead, by laboratory evolution we awakened latent, native enzyme activities to form the core of the RuMP cycle. We obtained fast methylotrophic growth in which ∼17 % of biomass was strictly obtained from methanol. This work lays the foundation for developing a versatile thermophilic bioproduction platform based on renewable methanol.

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Parageobacillus thermoglucosidasius strain:DSM 2542 | isolate:DSM 2542

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