Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification and characterization of small molecule inhibitors of the LINE-1 retrotransposon endonuclease

ABSTRACT: Identification and characterization of small molecule inhibitors of the LINE-1 retrotransposon endonuclease

PROVIDER: PRJNA1022033 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26205243_1.fastq.gz	Fastqsanger.gz
	SRR26205243_2.fastq.gz	Fastqsanger.gz
	SRR26205244_1.fastq.gz	Fastqsanger.gz
	SRR26205244_2.fastq.gz	Fastqsanger.gz
	SRR26205245_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Identification and characterization of small molecule inhibitors of the LINE-1 retrotransposon endonuclease

Project description:The long interspersed nuclear element-1 (LINE-1 or L1) retrotransposon is the only active autonomously replicating retrotransposon in the human genome. L1 harms the cell by inserting new copies, generating DNA damage, and triggering inflammation. Therefore, L1 inhibition could be used to treat many diseases associated with these processes. Previous research has focused on inhibition of the L1 reverse transcriptase due to the prevalence of well-characterized inhibitors of related viral enzymes. Here we present the L1 endonuclease as another target for reducing L1 activity. We have characterized structurally diverse small molecule endonuclease inhibitors using computational, biochemical, and biophysical methods. We also show that these inhibitors reduce L1 retrotransposition, L1-induced DNA damage, and inflammation reinforced by L1 in senescent cells. These inhibitors could be further used to better understand the life cycle of this element and its impact on human health.

2023-11-17 | GSE244265 | GEO

Tpl-2 and MEK small molecule inhibitors

Project description:Tpl-2 and MEK small molecule inhibitors

| PRJNA328166 | ENA

Small Molecule PCSK9 Inhibitors: Hit to lead optimization

Project description:Small Molecule PCSK9 Inhibitors: Hit to lead optimization

| PRJNA433685 | ENA

Identification of covalent small-molecule inhibitors of STING

Project description:Identification of covalent small-molecule inhibitors of STING

| PRJNA454500 | ENA

Identification of covalent small-molecule inhibitors of STING

Project description:Identification of covalent small-molecule inhibitors of STING

2018-05-02 | GSE113933 | GEO

Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen

Project description:Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen.

2015-01-01 | GSE17424 | GEO

PolyA-enriched RNA-seq of primary human hepatocyte spheroids treated with small molecule inhibitors targeting TEAD

Project description:TEAD1 emerged as an estrogen-sensitive gene that was upregulated in a HFD mouse model and in NAFLD patients. We investigated the roles of TEAD in NAFLD by treating primary human hepatocyte spheroids with two small molecule inhibitors blocking the TEAD/YAP interaction. One small molecule blocks the interaction by inhibiting TEAD autopalmitoylation (TEADap), the second small molecule blocks the interaction by binding to the surface of the TEAD protein (TEADsf).

2024-02-22 | E-MTAB-13207 | biostudies-arrayexpress

Determinants for DNA target structure selectivity of the human LINE-1 retrotransposon endonuclease.

Project description:The human LINE-1 endonuclease (L1-EN) is the targeting endonuclease encoded by the human LINE-1 (L1) retrotransposon. L1-EN guides the genomic integration of new L1 and Alu elements that presently account for approximately 28% of the human genome. L1-EN bears considerable technological interest, because its target selectivity may ultimately be engineered to allow the site-specific integration of DNA into defined genomic locations. Based on the crystal structure, we generated L1-EN mutants to analyze and manipulate DNA target site recognition. Crystal structures and their dynamic and functional analysis show entire loop grafts to be feasible, resulting in altered specificity, while individual point mutations do not change the nicking pattern of L1-EN. Structural parameters of the DNA target seem more important for recognition than the nucleotide sequence, and nicking profiles on DNA oligonucleotides in vitro are less well defined than the respective integration site consensus in vivo. This suggests that additional factors other than the DNA nicking specificity of L1-EN contribute to the targeted integration of non-LTR retrotransposons.

| S-EPMC1950540 | biostudies-literature

Novel method for multiplexed full-length single-molecule sequencing of the human mitochondrial genome

Project description:Here we describe a method to target, multiplex and sequence full-length, native single-molecule the human mitochondrial genome utilizing the RNA-guided DNA endonuclease Cas9.

| EGAS00001006203 | EGA

Small-Molecule PAPD5 Inhibitors Restore Telomerase Activity in Patient Stem Cells

Project description:Small-Molecule PAPD5 Inhibitors Restore Telomerase Activity in Patient Stem Cells

| PRJNA605628 | ENA