Project description:Placental gene expression in pregnancies established after the transfer of day 7 blastocysts derived from in vitro (IVP), somatic cell nuclear transfer (SCNT) and in vivo (AI) embryos
Project description:MeDIP-chip from livers of cloned and not cloned cattle at two stages (perinatal period, adulthood), using anti-methylcytosine antibody.
| PRJNA296015 | ENA
Project description:Cattle embryo production using Somatic Cell Nuclear Transfer
Project description:Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium
Project description:Trichostatin A does not correct specific errors of somatic cell nuclear transfer on the transcriptomic level highlighing the non-random nature of oocyte-mediated reprogramming errors.
Project description:Heat stress is a critical environmental factor that adversely affects the health and productivity of cattle. It leads to substantial declines in productivity and compromises immune function, thereby increasing susceptibility to disease. Crossbred cattle (Bos indicus × Bos taurus) are known for their enhanced productivity. However, they are relatively vulnerable to environmental stressors. In recent years, the intensification of global warming and associated climatic extremes has further heightened their risk of heat stress. In this context, the present study investigated the underlying biological responses to heat stress through genome-wide expression analysis in Vrindavani crossbred cattle. The results identified sets of genes activated during heat stress and subsequent functional characterization revealed key molecular mechanisms of thermal stress response in these animals.
Project description:Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient because of aberrant genomic reprogramming. In addition to random reprogramming errors, we hypothesized the presence of specific errors as evidenced by common anomalies among clones. We found that Xist, which normally inactivates one of the two X chromosomes in females, was ectopically expressed from the active X (Xa) chromosome in cloned mouse embryos of both sexes. Deletion of Xist on Xa normalized global gene expression and produced about a 10-fold increase in cloning efficiency. We also identified an Xist-independent mechanism that specifically downregulated a subset of X-linked genes through somatic-type repressive histone blocks. Thus, we have identified nonrandom reprogramming errors in mouse cloning, which provide promising targets for breakthroughs in SCNT cloning technology. Gene expression were measured in mouse in vitro fertilized and somatic cell cloned blastocysts. More than three biological replicates were performed in each group using defferent nuclear donor cells.