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ABSTRACT: RNA sequencing of epithelium from the earthworm, Dendrobaena veneta (European nightcrawler)

PROVIDER: PRJNA1026068 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26322680_1.fastq.gz	Fastqsanger.gz
	SRR26322680_2.fastq.gz	Fastqsanger.gz
	SRR26322681_1.fastq.gz	Fastqsanger.gz
	SRR26322681_2.fastq.gz	Fastqsanger.gz
	SRR26322682_1.fastq.gz	Fastqsanger.gz

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Dendrobaena veneta

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De novo RNA sequencing of the gut and cerebral ganglion of Dendrobaena veneta exposed to carbamazepine

Project description:De novo RNA sequencing of the gut and cerebral ganglion of Dendrobaena veneta exposed to carbamazepine

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Mixed baits reduce the oxidative stress of common carp (Cyprinus carpio): Based on microRNA sequencing

Project description:Purpose:In order to elucidate the molecular mechanism of the effects of different diets on the antioxidant capacity of fish, three groups of adult common carp (Cyprinus carpio) (initial weight 517.8 ±50 g) were fed with earthworm, earthworm + duckweed and duckweed, respectively, which were performed by miRNA sequencing. Methods:MiRNA profiles of common carp fed with earthworm, earthworm + duckweed and duckweed were generated by deep sequencing, in duplicate, using Illumina Hiseq 2500 platform. qRT–PCR validation was performed using SYBR Green assays Results: We built microRNA libraries by using total RNA and contained filtered 20,868,000, 18,984,242, 22,300,487, 19,538,683, 17,110,676 and 14,747,090 high-quality reads that were obtained from sample A1, A2, M1, M2, P1 and P2 respectively.In total, we discovered 236 known carp miRNAs (225, 225, 222, 227, 228 and 219 in A1, A2, M1, M2, P1and P2, respectively).For the known miRNA, 9 differentially expressed miRNAs (DEM) were found between A and M and 8 known miRNAs differentially expressed between P and M, and 11 of these were validated with qRT–PCR. The expression level of 11 known microRNAs via miRNA-seq had highly similar with the relative expression level of 11 known microRNAs via qRT-PCR. Conclusions: In present study, we demonstrated the transcriptional profiles, identification of 5 differentially expressed miRNAs be reported related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p and miR-125b-5p. For omnivorous fish, feed a single bait may might cause oxidative stress inordinately.

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