Project description:Over the last 20 years, the advances in sequencing technologies highlighted the unique composition of the salivary glands of blood-feeding arthropods. Further biochemical and structural data demonstrated that salivary proteins can disrupt host hemostasis, inflammation and immune response in addition to favor pathogen transmission. Previously, a Sanger-based sialome of the adult Ochlerotatus. triseriatus female salivary glands was published based on 731 ESTs. Here we revisited O. triseriatus salivary glands contents using an Illumina-sequencing approach of both male and female tissues. In the current data set we report 10,317 coding DNA sequences classified into several functional classes. The translated transcripts also served as reference database for the proteomic analysis of O. triseriatus female saliva, in which unique peptides of 101 proteins were found. Finally, comparison of male and female libraries allowed the identification of female-enriched transcripts that are potentially related to blood acquisition and virus transmission.
2022-10-15 | PXD033588 | Pride
Project description:Illumina sequencing data for composite letter DNA storage
Project description:The purpose of this study was to measure DNA methylation and siRNA expression across the maize genome. The experimental data was derived from shotgun bisulfite sequencing, siRNA sequencing, and mRNA sequencing (Illumina, single end for all three)
Project description:We identify m6dA in human genomic DNA by DNA immunoprecipitation and subsequent Hi-Throughput Illumina sequencing (DIP-Seq). We use a dA6m specific antibody for DNA pulldown, and input as control. In addition, we performed RNA High Throughput Illumina Sequencing on biologically matched samples. This data supplements our analysis of already published data.
Project description:Bisulfite conversion and whole genome-single base next generation sequencing of DNA from a single iPSC clone (CMC28). This method provides exceptional depth of the sequenced methylome. Bisulfite converted DNA from a single iPSC clone (CMC28), and get its high-throughput sequence data with Illumina.