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Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function

ABSTRACT: Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function

PROVIDER: PRJNA1027458 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR32702210_1.fastq.gz	Fastqsanger.gz
	SRR32702210_2.fastq.gz	Fastqsanger.gz
	SRR32702211_1.fastq.gz	Fastqsanger.gz
	SRR32702211_2.fastq.gz	Fastqsanger.gz
	SRR32702212_1.fastq.gz	Fastqsanger.gz

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Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [ATAC-Seq]

Project description:Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [ATAC-Seq]

| PRJNA1027470 | ENA

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [RNA-Seq]

Project description:Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [RNA-Seq]

| PRJNA1027473 | ENA

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [CUT&Run]

Project description:Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [CUT&Run]

| PRJNA1236400 | ENA

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [CUT&Run]

Project description:Genes encoding subunits of the BAF (mammalian SWI/SNF) ATP-dependent chromatin remodeling complex are among the those most enriched for deleterious de novo mutations in intellectual disabilities and autism spectrum disorder, but the causative molecular pathways are not understood. Synaptic activity in neurons is critical for learning and memory and proper neural development. While BAF is required for activity-dependent developmental processes, such as dendritic outgrowth, the immediate molecular consequences of neuronal activity on BAF complexes are unknown. Here we report that neuronal activity induces dramatic remodeling of the subunit composition of BAF complexes within 15 minutes, concurrent with both phosphorylation and dephosphorylation of its subunits. These biochemical effects are a convergent phenomenon downstream of multiple calcium-activated signaling pathways in neurons and fibroblasts and correspond to changes in BAF-dependent chromatin accessibility. Our studies imply that BAF decodes signals at the membrane by altering the combinatorial composition of its subunits.

2025-03-15 | GSE292056 | GEO

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [ATAC-Seq]

2025-03-15 | GSE245254 | GEO

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function [RNA-Seq]

2025-03-15 | GSE245251 | GEO

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-03-15 | GSE245256 | GEO

Acute BAF perturbation causes immediate changes in chromatin accessibility

Project description:Acute BAF perturbation causes immediate changes in chromatin accessibility

| PRJNA623383 | ENA

Acute BAF perturbation causes immediate changes in chromatin accessibility

Project description:Loss-of-function mutations in genes coding for subunits of the large, multifarious BRG1/BRM associated factor (BAF) chromatin remodeling complexes are frequently causative for cancer or developmental diseases1-5. Cells lacking the most frequently mutated subunits like the ATPase SMARCA4 typically exhibit drastic chromatin accessibility changes, especially of important regulatory regions6-12. However, so far it remains unknown how these changes are established over time, and whether they are causative for intra-complex synthetic lethalities abrogating the formation (SMARCC1-SMARCC2)8,13,14 or activity (SMARCA4-SMARCA2)15-17 of BAF complexes. Here, we utilize the dTAG system18 to induce acute degradation of BAF subunits in wild-type and BAF mutant backgrounds and analyze the resulting chromatin accessibility changes with high kinetic resolution. We observe that chromatin alterations are established faster than the duration of one cell cycle and that maintaining genome accessibility requires constant ATP-dependent remodeling. Completely abolishing BAF complex function by acute degradation of a synthetic lethal subunit in a paralog-deficient background results in a near-complete loss of chromatin accessibility at BAF-controlled sites, especially at super-enhancers, providing a mechanism for intra-complex synthetic lethalities.

2020-12-23 | PXD018381 | Pride

Acute BAF perturbation causes immediate changes in chromatin accessibility

Project description:Loss-of-function mutations in genes coding for subunits of the large, multifarious BRG1/BRM associated factor (BAF) chromatin remodeling complexes are frequently causative for cancer or developmental diseases. Cells lacking the most frequently mutated subunits, like the ATPase SMARCA4, typically exhibit drastic chromatin accessibility changes, especially of important regulatory regions. However, so far it remains unknown how these changes are established over time, and whether they are causative for intra-complex synthetic lethalities abrogating the formation or activity of BAF complexes. Here, we utilize the dTAG system to induce acute degradation of BAF subunits in wildtype and BAF mutant backgrounds and analyze the resulting chromatin accessibility changes with high kinetic resolution. We observe that chromatin alterations are established faster than the duration of one cell cycle and that maintaining genome accessibility requires constant ATP-dependent remodeling. Completely abolishing BAF complex function by acute degradation of a synthetic lethal subunit in a paralog-deficient background results in a near-complete loss of chromatin accessibility at BAF-controlled sites, especially at super-enhancers, providing a mechanism for intra-complex synthetic lethalities.

2020-12-22 | GSE148175 | GEO

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