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Study of MED1 acetylation in ER positive breast cancer cells [HiCAR-seq]

ABSTRACT: Study of MED1 acetylation in ER positive breast cancer cells [HiCAR-seq]

PROVIDER: PRJNA1030306 | ENA |

REPOSITORIES: ENA

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Study of MED1 acetylation in ER positive breast cancer cells [HiCAR-seq]

Project description:Chromatin occupancy of MED1 and other Mediator components, as well as Pol II, were studied in MCF7 cells. MED1 acetylation affects chromatin occupancy of its related factors, which was analyzed with CUT&RUN-seq, ChIP-seq and sequential ChIP-seq assay (by pan-acetyl-lysine antibody) with MED1 KR mutant (HA tag) where acetylation residues were replaced. Combined with RNA seq analysis for gene expression in MCF7 cells with MED1 KO and KR/KQ MED1 ectopic expression, the role of MED1 acetylation in gene transcription control was investigated.

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Project description:Study of MED1 acetylation in ER positive breast cancer cells [CUT&Run]