Project description:Timber Tracking Project IPB-WRI: Collection of physical timber reference material and setting up a reference data building pipeline for DNA of commercial timber species (Diospyros celebica. Bakh)
Project description:Wood density is a foundamental quality trait for structural timber, bioenergy and pulp industries. We investigated genes differentially transcribed in radiate pine juvneile trees with distinct wood density using cDNA microarrays.
Project description:Bacteria from anaerobic Microcolony-seq condition that were kept at the -80C were regrown in liquid LB at 37C for 5 hours. DNA was extracted from 1 mL of bacteria using the DNeasy Blood & Tissue Kit (Qiagen). Library preparation and sequencing was carried out by the Genomics Applications Laboratory, Core Research Facility, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel. DNA libraries for sequencing were prepared using the kit (ILMN DNA LP (M) Tagmentation (24 Samples, IPB) 20060060) (Illumina, Inc., USA) according to manufacturing instructions. At least 100X sequencing depth for each nucleotide in each sample was targeted. Samples were deep-sequenced with the Nextseq500 machine using the 150-cycle mid output kit (Illumina, Inc., USA) from the forward read direction.
Project description:Wood stiffness is the most important wood quality trait of forest trees for structural timber production. We investigated genes differentially transcribed in radiate pine trees with distinct wood stiffness using bulked segregant analysis (BSA) and cDNA microarrays. Transcript accumulation in earlywood (EW) and latewood (LW) of high (HS) and low stiffness (LS) trees in two progeny trials was compared.
Project description:This SuperSeries is composed of the following subset Series: GSE22171: Pacific salmon gill samples: fate tracking in river, sampled in ocean GSE22177: Pacific salmon gill samples: fate tracking in river GSE22347: Pacific salmon gill samples: fate tracking at spawning grounds Refer to individual Series