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The H2Bub1-deposition complex is required for human and mouse cardiogenesis (RNA-Seq)

ABSTRACT: The H2Bub1-deposition complex is required for human and mouse cardiogenesis (RNA-Seq)

PROVIDER: PRJNA1032897 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26552100_1.fastq.gz	Fastqsanger.gz
	SRR26552100_2.fastq.gz	Fastqsanger.gz
	SRR26552101_1.fastq.gz	Fastqsanger.gz
	SRR26552101_2.fastq.gz	Fastqsanger.gz
	SRR26552102_1.fastq.gz	Fastqsanger.gz

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The H2Bub1-deposition complex is required for human and mouse cardiogenesis (ChIP-Seq)

Project description:De novo variants affecting monoubiquitination of histone H2B (H2Bub1) are enriched in human congenital heart disease. H2Bub1 is required in stem cell differentiation, cilia function, post-natal cardiomyocyte maturation, and transcriptional elongation. However, how H2Bub1 affects cardiogenesis is unknown. We show that the H2Bub1-deposition complex (RNF20-RNF40-UBE2B) is required for mouse cardiogenesis and for differentiation of human iPSCs into cardiomyocytes. Mice with cardiac-specific Rnf20 deletion are embryonic lethal and have abnormal myocardium. We then analyzed H2Bub1 marks during differentiation of human iPSCs into cardiomyocytes. H2Bub1 is erased from most genes at transition from cardiac mesoderm to cardiac progenitor cells, but is preserved on a subset of long cardiac-specific genes. When H2Bub1 is reduced in iPSC-cardiomyocytes, long cardiac-specific genes have fewer full-length transcripts. This correlates with H2Bub1 accumulation near the center of these genes. H2Bub1 accumulation near the center of tissue-specific genes was also observed in embryonic fibroblasts and fetal osteoblasts. In summary, we show that normal H2Bub1 distribution is required for cardiogenesis and cardiomyocyte differentiation, and point to H2Bub1 regulating tissue-specific gene expression by increasing the amount of full-length transcripts.

2023-10-31 | GSE246410 | GEO

The H2Bub1-deposition complex is required for human and mouse cardiogenesis (RNA-Seq)

2023-10-31 | GSE246411 | GEO

The H2Bub1-deposition complex is required for human and mouse cardiogenesis

Project description:The H2Bub1-deposition complex is required for human and mouse cardiogenesis

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The H2Bub1-deposition complex is required for human and mouse cardiogenesis (ChIP-Seq)

Project description:The H2Bub1-deposition complex is required for human and mouse cardiogenesis (ChIP-Seq)

| PRJNA1032889 | ENA

The H2Bub1-deposition complex is required for human and mouse cardiogenesis.

Project description: Not available

| S-EPMC10730087 | biostudies-literature

The H2Bub1-deposition complex is required for human and mouse cardiogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-10-31 | GSE246413 | GEO

Transcriptom modulated by LDOC1 / H2Bub1 axis

Project description:Monoubiquitination of histone H2B on lysine 120 (H2Bub1) is one of the prominent epigenetic marks that regulates multiple cellular processes, including immunity, stem cell differentiation, tumorigenesis, and DNA damage response, mainly through affecting transcription of specific gene sets. The Ring Finger Protein 40 (RNF40) and its paralog RNF20 forms a stable E3 ligase heterodimer is the major enzymatic complex responsible for H2B monoubiquitylation. Dysregulation of H2Bub1 was observed in early stage of non-small cell lung carcinoma (NSCLC). However, the underlying regulatory mechanism and the impact of aberrant H2Bub1 level in the metastasis and chemotherapy response of NSCLC patients remains unelucidated. We found LDOC1 associated with RNF40 in NSCLC A549 cells. In this study, we investigate the role of LDOC1 in epigenome and aggressiveness of NSCLC involving H2Bub1.

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A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis

Project description:Large intergenic non-coding RNAs (lincRNAs) play widespread roles in epigenetic regulation during multiple differentiation processes, but little is known about their mode of action in cardiac differentiation. Here, we identified the key roles of a lincRNA, termed linc1405, in modulating the core network of cardiac differentiation by functionally interacting with Eomes. Chromatin- and RNA- immunoprecipitation assays showed that exon2 of linc1405 physically mediates a complex consisting of Eomes, Trithorax group (TrxG) subunit WDR5, and histone acetyltransferase GCN5 binding at the enhancer region of Mesp1 gene and activates its expression during cardiac mesoderm specification of embryonic stem cells. Importantly, linc1405 co-localizes with Eomes, WDR5, and GCN5 at the primitive streak and linc1405 depletion impairs heart development and function in vivo. In summary, linc1405 mediates a Eomes/WDR5/GCN5 complex that contributes to cardiogenesis, highlighting the critical roles of lincRNA-based complexes in the epigenetic regulation of cardiogenesis in vitro and in vivo.

2018-02-27 | GSE111132 | GEO

A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis

Project description:A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis

| PRJNA435989 | ENA

BMAL1-TTK-H2Bub1 loop deficiency contributes to impaired BM-MSC-mediated bone formation in senile osteoporosis

Project description:We performed multiomic high-throughput sequencing to recapitulated the BMAL1-H2Bub1 loop in mesenchymal stem cells osteogenesis. We used lentiviruses to diminish RNF40 and WAC, which are parts of the obligate complex and monoubiquitinated histone H2B, in MSCs, and analysed the H2Bub1 and PolII CUT&Tag-seq.

2022-08-06 | GSE210425 | GEO

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