Project description:Pokkah boeng disease, caused by the fungal pathogen Fusarium sacchari, is a significant threat to sugarcane production worldwide. To elucidate the molecular defense mechanisms of sugarcane against this pathogen, we performed a comprehensive transcriptomic analysis using the hybrid cultivar ZZ1 (Saccharum officinarum x Saccharum spontaneum). Leaf tissues were collected at multiple time points (0, 12, 24, 48, 72, 120, and 144 hours) following inoculation with F. sacchari. Total RNA was extracted and sequenced using the Illumina Novaseq platform. This study aims to identify key differentially expressed genes and regulatory pathways involved in the sugarcane-pathogen interaction, providing valuable genomic resources for breeding disease-resistant sugarcane varieties.