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RNA sequence in alveolar macrophage of mice treated with HDM or Combined with PR8

ABSTRACT: RNA sequence in alveolar macrophage of mice treated with HDM or Combined with PR8

PROVIDER: PRJNA1033697 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26587961_1.fastq.gz	Fastqsanger.gz
	SRR26587961_2.fastq.gz	Fastqsanger.gz
	SRR26587962_1.fastq.gz	Fastqsanger.gz
	SRR26587962_2.fastq.gz	Fastqsanger.gz
	SRR26587963_1.fastq.gz	Fastqsanger.gz

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RNA sequence in alveolar macrophage of mice treated with HDM or Combined with PR8

Project description:To research the different expressive genes in alveolar macrophage during asthma excerbation, we build asthma excerbation mice use HDM combined with PR8. we sepreated and collected the alveolar macrophages in BALF of mice then test the RNA expression. in this data, LysMCre Cul5fl/fl mice also challenaged with HDM combined with PR8 to compared the different expressive genes in alveolar macrophage.

2023-11-05 | GSE246601 | GEO

Host response in cells from broncho-alveolar lavage (BAL) isolated from mice infected with influenza virus (PR8) and/or Staphylococcus aureus (Newman)

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Project description:In this report, we performed bulk RNA sequencing of AMs from AMs (alone), AM: BAL neutrophils (BAL), and AM: PR8 neutrophils (PR8) (1:1 ratio).

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Cell-sorted monoytes from PR8-infected mice

Project description:Primary monocytes from PR8-infected mice were magnetically sorted and processed for RNA-sequencing

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Transcriptional changes in alveolar macrophages from adults with asthma after intrabronchial allergen challenge.

Project description:In recent years, alveolar macrophages (AM) have been shown to play an important role in environmental allergen-induced airway inflammation in asthma. In this study, we investigated the effect of house dust mite (HDM) and lipopolysaccharide (LPS) challenge on the transcriptome of AM from patients with mild asthma. We have shown previously that intrabronchial HDM/LPS challenge induces a mixed eosinophilic and neutrophil airways inflammation in asthma patients(5). Therefore, we hypothesize that exposure of AM to HDM/LPS would upregulate genes associated with eosinophil and neutrophil signaling. AM were harvested from the bronchoalveolar fluid of 8 asthma patients who were challenged with either saline in one lung segment or HDM/LPS in the contralateral lung segment. We performed RNA sequencing and found over 1000 significantly different expressed genes following HDM/LPS challenge. To the best of our knowledge, this is the first investigation to examine gene expression profiles in AM from asthma patients challenged with HDM and LPS in vivo. Using a well-controlled study design allowing paired analysis of saline and HDM/LPS effects in the same patient, we identified different transcriptional profiles in AM as consequence of local exposure to HDM and LPS. These findings may provide a better understanding of AM functions in (exacerbations of) allergic asthma.

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Alveolar macrophage gene expression - PIKfyve knockout

Project description:comparison of Alveolar macrophge gene expression in wild type and PIKfyve ko We identifed gene that important for alveolar macrophage development

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BCR sequences in HDM-allergic patients

Project description:BCR sequences in HDM-allergic patients

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CD11b restricts alveolar macrophage activation during pneumococcal pneumonia and prevents excessive lung inflammation

Project description:Bacterial lung infections are associated with strong infiltration of CD11b+ myeloid cells, which limit life-threatening disease, but also severely damage lung tissue. In a murine lung infection model with Streptococcus pneumoniae, we found intrinsic upregulation of CD11b on resident alveolar macrophages. Such CD11b expression was associated with transcriptomic and proteomic adaptations by alveolar macrophages, leading to the identification of specific molecules and pathways that depended on CD11b. In the absence of CD11b, the antimicrobial defense of alveolar macrophages was strongly reduced, and the production of neutrophil-recruiting chemokines was more pronounced. Moreover, CD11b expression limited the infection and prevented excessive alveolar damage. In conclusion, our study provides detailed molecular insights into the alveolar macrophage-specific immune response to Streptococcus pneumoniae lung infection and reveals profound CD11b-dependent alterations that are critical for effective antimicrobial immunity, neutrophil recruitment, and prevention of alveolar damage.

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Cell-sorted monoytes from PR8-infected mice

Project description:Cell-sorted monoytes from PR8-infected mice

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Project description:In our lower respiratory tract, the alveolar spaces are divided from the bloodstream and the external environment by only a few microns of interstitial tissue. Alveolar macrophages (AMs) defend this delicate mucosal surface from invading infections by regularly patrolling the site. AMs have three behaviour modalities to achieve this goal: extending cell protrusions to sample surrounding areas, squeezing the whole cell body between alveoli, and patrolling by moving the cell body around each alveolus. In this study we found Rho GTPase, cell division control protein 42 (CDC42) expression significantly decreased after berry-flavoured e-cigarette (e-cig) exposure. This resulted in a shift in AM behaviour from squeezing to probing. Changes in AM behaviour led to a reduction in the clearance of inhaled bacteria, Pseudomonas aeruginosa. These findings shed light on pathways involved in AM migration and highlight the harmful impact of e-cigarette vaping on alveolar macrophage function.

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