Project description:In the present study, we used NGST to characterize mRNA-seq of control-, moderate hypoxia-treated and severe hypoxia-treated Micropterus salmoides livers to elucidate the molecular mechanisms of hypoxia adaptation. This is the first report on integrated analysis of the tissue specific and temporal changes in gene expression in largemouth bass (Micropterus salmoides) exposed to hypoxia could reveal mechanisms of hypoxia adaptation. We provide a good case study with which to analyse mRNA expression and profile non-model fish species using NGST.
Project description:Zearalenone (ZEN), a prevalent Fusarium-derived mycotoxin in plant protein-based aquafeeds, poses severe threats to farmed aquatic animals. However, its toxic effects and underlying mechanisms on the commercially critical largemouth bass (Micropterus salmoides) remain largely uncharacterized. Here, juvenile M. salmoides were fed diets containing 0, 100, and 1000 μg/kg ZEN for 28 days. Growth performance, hepato-intestinal histopathology, barrier function, and antioxidant/biochemical parameters were systematically determined, combined with 16S rRNA sequencing and untargeted metabolomics to elucidate ZEN’s toxic mechanism. Dietary ZEN induced biphasic growth dysregulation: low-dose ZEN significantly promoted fish growth, while high-dose ZEN impaired feed efficiency, caused severe oxidative damage, histopathological lesions, and tight junction gene downregulation in the liver and intestine. ZEN exposure drastically disrupted intestinal microbial homeostasis, reduced core commensal bacteria, enriched potential pathogens, and disturbed host metabolic pathways related to nutrient transport, amino acid/lipid metabolism, and inflammatory response. This study revealed that ZEN induced growth dysregulation and hepato-intestinal injury in M. salmoides via disrupting gut microbial and metabolic homeostasis, providing novel insights into ZEN toxicology in carnivorous fish and theoretical support for mycotoxin control in aquaculture.
Project description:We sequenced mRNA from 9 liver samples of juvenile largemouth bass (Micropterus salmoides) taken from different lead concentration exposure treatment fish and control fish to investigate the transcriptome and comparative expression profiles of largemouth bass liver undergoing lead exposure.
2019-01-12 | GSE124986 | GEO
Project description:Micropterus salmoides plant protein 16S
| PRJNA956853 | ENA
Project description:Micropterus salmoides oxidized fish oil 16s
