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Nanopore long-read RNA sequencing shows region-specific sex differences in wild-type mouse brain mRNA isoform expression and usage

ABSTRACT: Nanopore long-read RNA sequencing shows region-specific sex differences in wild-type mouse brain mRNA isoform expression and usage

PROVIDER: PRJNA1034151 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26622700_1.fastq.gz	Fastqsanger.gz
	SRR26622800_1.fastq.gz	Fastqsanger.gz
	SRR26622900_1.fastq.gz	Fastqsanger.gz
	SRR26622701_1.fastq.gz	Fastqsanger.gz
	SRR26622801_1.fastq.gz	Fastqsanger.gz

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Nanopore long-read RNA sequencing shows region-specific sex differences in wild-type mouse brain mRNA isoform expression and usage

Project description:To address differences in splicing across brain regions (cerebellum, cortex, hippocampus, and striatum) and sexes, we used long-read Oxford Nanopore Technologies (ONT) RNA sequencing to sequence 40 wild-type mouse brain cDNA libraries from 10 mice and calculated differential expression and transcript usage. We found that there is differential gene expression, differential transcript expression, and differential transcript usage across all brain regions. We found that the brain region with the most differential expression and transcript usage is the cerebellum, potentially driven by differences in cell type composition. Additionally, our findings suggest there is much differential splicing across brain regions and to a lesser extent, within brain regions across sexes.

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