Project description:This project aims to identify neuronal signatures in the hippocampus of Home Cage WT mice. For this, we sorted neuronal and non neuronal nuclei (NeuN+ and NeuN- ) of total hippocampus from 5-month-old WT mice. ChIP experiments were performed for 2 different histone marks (H3K27me3 and H3K27ac) in about 4-500000 sorted NeuN+ and NeuN- nuclei. H3K27ac signatures were also assessed in 12 month-old WT mice. We have INPUT DNA For each condition (NeuN+ and NeuN-).
Project description:Alpha-herpesviruses establish a life-long infection in the nervous system of the affected host; while this infection is restricted to peripheral neurons in a heathy host, it can spread within the neuronal circuitry in compromised individuals leading to adverse health consequences. Pseudorabies virus (PRV) an alpha-herpesvirus, requires the viral protein Us9 for sorting virus particles into axons. It does so by mediating an interaction of virus particles with neuronal transport machinery. Us9-mediated axonal sorting also depends on the state of neuronal maturation as the proteome composition changes with neuronal development of dendrites and axons. Immature superior cervical ganglia (SCGs) have rudimentary neurites that lack markers of mature dendrites or axons. Immature SCGs can be infected by PRV, but show markedly reduced Us9-dependent sorting into neurites. Mature SCGs abundantly express a variety of proteins characteristic of vesicle-transport machinery. Proteomics studies identified several novel Us9-associated neuronal proteins with potential roles in the regulation of axonal sorting and subsequent anterograde spread of virus particles in axons. For example, we found that SMPD4/n-sMase3, a sphingomyelinase abundant in lipid-rafts, associates with Us9 and is a negative regulator of axonal mediated spread of PRV, a potential novel antiviral function.
Project description:H3K27ac ChIP-Seq data of the T-ALL cell line LOUCY was obtained to find active regions in the genome and to correlate that with expression profiling of lncRNAs in the LOUCY cell line. ChIP-sequencing data was generated for H3K27ac in the T-ALL cell line LOUCY.
Project description:This data set is for the article:
Endothelial-neuronal coupling revealed in a decoupled neurovascular unit based Organ-on-Chip approach.