Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1034815 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR26638100.fastq.gz | Fastqsanger.gz | |||
| SRR26638101.fastq.gz | Fastqsanger.gz | |||
| SRR26638102.fastq.gz | Fastqsanger.gz | |||
| SRR26638103.fastq.gz | Fastqsanger.gz | |||
| SRR26638104.fastq.gz | Fastqsanger.gz |
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Project description:Sex-steroids concentrations throughout the estrous cycle modulate endometrial function and fertility in cattle. The objective of this study was to compare the post-estrus endometrial transcriptome of cows that were exposed to contrasting pre-estrus concentrations of progesterone before they displayed estrus and ovulated spontaneously. We hypothesized that greater pre-estrus concentration of progesterone alters post-estrus endometrial function even after the display of estrus and spontaneous ovulation.
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Project description:This study relates to pregnancy outcome after assisted reproduction of fertility-classified cattle. The aim is to investigate how the uterine environment impacts and programs conceptus survival and development. The study found that ripple effects of dysregulated conceptus-endometrial interactions elicit post-elongation pregnancy loss in subfertile animals during the implantation period.
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Project description:In both beef and dairy cattle, the majority of embryo loss occurs in the first 14 days following insemination. During this period, the embryo is completely dependent on its maternal uterine environment for development, growth and ultimately survival, therefore an optimum uterine environment is critical to embryo survival. We used microarrays to assess endometrial gene expression in high and low fertility heifers during the mid-luteal phase of the estrous cycle. Charlaois × Limousin heifers were artificially inseminated on 4 successive occasions and heifers were subsequently characterized as either high or low fertility (HF; LF) based on the presence of an embryo on day 28 of pregnancy on all four inseminations or on one occasion only, respectively. From this population of animals, HF and LF heifers were slaughtered on day 7 of a synchronized estrous cycle and global gene expression in uterine endometrial tissue was determined using the Affymetrix 23K Bovine GeneChip.
2011-06-10 | E-GEOD-29853 | biostudies-arrayexpress
Project description:In both beef and dairy cattle, the majority of embryo loss occurs in the first 14-16 days following insemination. During this period, the embryo is completely dependent on its maternal uterine environment for development, growth and ultimately survival, therefore an optimum uterine environment is critical to embryo survival. We used microarrays to assess endometrial gene expression in high and low fertility heifers during the late-luteal phase of the estrous cycle. Charlaois M-CM-^W Limousin heifers were artificially inseminated on 4 successive occasions and heifers were subsequently characterized as either high or low fertility (HF; LF) based on the presence of an embryo on day 28 of pregnancy on all four inseminations or on one occasion only, respectively. From this population of animals, HF and LF heifers were slaughtered on day 14 of a synchronized estrous cycle and global gene expression in uterine endometrial tissue was determined using the AffymetrixM-BM-. 23K Bovine GeneChip. Array Annotation link: http://mad-db.science.uva.nl/~wdeleeuw/HybridAnnot/
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Project description:Over the past three decades, there has been a significant decline in dairy cattle fertility. A large proportion of pregnancy losses are believed to occur during the pre-implantation period, when the developing embryo is elongating rapidly and signaling its presence to the maternal system. The molecular mechanisms that prevent progression of the estrous cycle and allow the allogenic embryo to survive within the maternal environment are not well understood. To gain a more complete picture of these molecular events, global transcriptional profiling was performed using endometrial tissues from reproductive day 17 pregnant and non-pregnant (cycling) Holstein-Friesian dairy cattle. 22 animals were enrolled in this study, 2 samples from each animal were used for microarray analysis consisting of caruncular and intercaruncular endometrium. A reference design was used for hybridisation, whereby each of the 44 samples were hybridised to individual microarrays along with a pooled reference sample. The reference sample was generated by taking equal concentrations of each of the experimental samples.
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