Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1035444 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Two Arabidopsis thaliana splicing factor [AtU2AF65 isoforms (AtU2AF65a and AtU2AF65b)] mutants displayed the opposite flowering phenotypes. To assay the RNA processing including alternative splicing and pre-mRNA splicing of target genes of this protein in Arabidopsis, the 7-day seedlings (shoot apices) of wild type atu2af65a and atu2af65b mutants were used for RNA-Seq.
2023-05-01 | E-MTAB-12648 | biostudies-arrayexpress
Project description:PLETHORA transcription factors (PLTs) are master regulators of plant development. In Arabidopsis, loss of shoot meristematic PLTs leads to destabilised phyllotaxis, but whether these factors have similar functions in more distantly related species, is not known. Here, we investigated the role of PLTs in shaping cucumber shoot architecture. Through transcriptional analysis, we determined that two of the four homologs - CsPLT3/7 and CsPLT5 - are expressed in complementary domains in the shoot apex. In situ hybridisation showed that whereas CsPLT3/7 was expressed in the shoot apical meristem summit and leaf axils, CsPLT5 transcripts localised beneath the CsPLT3/7 domain and to the adaxial side of leaf primordia. Both transcription factors recognised an ANT-like consensus motif and bound to SAM organising genes in vitro. Using a panel of TILLING mutants of each of the four CsPLT homologs, we only identified strong phenotypic defects for Csplt3/7 mutants. The combination of Csplt1/2 and Csplt3/7 proved embryonic lethal. In young seedlings, loss of CsPLT3/7 caused absence of a shoot apical meristem, which regenerated de novo shoot from a flat shoot apex during subsequent seedling development. Mature Csplt3/7 shoots exhibited defects in internode length stabilisation, revealing both extremely short and long internodes, stem architecture, flower morphology, and axillary organ initiation. Moreover, phyllotactic patterns were slightly shifted from a spiral towards a distichous orientation. We thus provide the first evidence of a role for PLTs in shoot development outside of Arabidopsis.
2026-01-19 | GSE294326 | GEO
Project description:PLETHORA transcription factors (PLTs) are master regulators of plant development. In Arabidopsis, loss of shoot meristematic PLTs leads to destabilised phyllotaxis, but whether these factors have similar functions in more distantly related species, is not known. Here, we investigated the role of PLTs in shaping cucumber shoot architecture. Through transcriptional analysis, we determined that two of the four homologs - CsPLT3/7 and CsPLT5 - are expressed in complementary domains in the shoot apex. In situ hybridisation showed that whereas CsPLT3/7 was expressed in the shoot apical meristem summit and leaf axils, CsPLT5 transcripts localised beneath the CsPLT3/7 domain and to the adaxial side of leaf primordia. Both transcription factors recognised an ANT-like consensus motif and bound to SAM organising genes in vitro. Using a panel of TILLING mutants of each of the four CsPLT homologs, we only identified strong phenotypic defects for Csplt3/7 mutants. The combination of Csplt1/2 and Csplt3/7 proved embryonic lethal. In young seedlings, loss of CsPLT3/7 caused absence of a shoot apical meristem, which regenerated de novo shoot from a flat shoot apex during subsequent seedling development. Mature Csplt3/7 shoots exhibited defects in internode length stabilisation, revealing both extremely short and long internodes, stem architecture, flower morphology, and axillary organ initiation. Moreover, phyllotactic patterns were slightly shifted from a spiral towards a distichous orientation. We thus provide the first evidence of a role for PLTs in shoot development outside of Arabidopsis.
2026-01-19 | GSE294327 | GEO
Project description:Inducible overexpression of Arabidopsis meristem regulators by AlcR / AlcA system. Plants harboring 35S::AlcR/AlcA::GOI (GUS control, LEAFY, SHOOTMERSTEMLESS, WUSCHEL)constructs were grown in continous light for 12 days and induced with 1% Ethanol. After 12h of EtOH treatment, seedlings were dissected and RNA was processed from the shoot apex and young leaves. Affymetrix Ath1 arrays were hybridized in duplicates from each experiment.
2006-01-01 | E-MEXP-432 | biostudies-arrayexpress
Project description:We identified PRP4 kinase-A (PRP4ka) in a forward genetic screen based on an alternatively-spliced GFP reporter gene in Arabidopsis thaliana (Arabidopsis). Prp4 kinase, which was the first spliceosome-associated kinase shown to regulate splicing in fungi and mammals, has not yet been studied in plants. Analysis of RNA-seq data from the prp4ka mutant revealed widespread perturbations in alternative splicing. A quantitative iTRAQ-based phosphoproteomics investigation of the mutant identified phosphorylation changes in several serine/arginine-rich proteins, which regulate constitutive and alternative splicing, as well as other splicing-related factors. The results demonstrate the importance of PRP4ka in alternative splicing and suggest that PRP4ka may influence alternative splicing patterns by phosphorylating a subset of splicing regulators.
2018-10-25 | PXD008580 | Pride
Project description:Gene expression in the shoot apex of 3-week old Arabidopsis thaliana (ecotype Col-0) was compared to DRB2 (AT2G28380) null mutants.
2014-07-01 | E-MTAB-2586 | biostudies-arrayexpress