Project description:Obesity is predicted to become the largest modifiable risk factor for breast cancer in postmenopausal women, yet the mechanisms underlying this association are unclear. We identified a novel protective mechanism for lean adipocytes to suppress breast cancer by secreting the oxylipin 9S-HODE, which induces ferroptosis in breast cancer cells while sparing normal breast epithelial cells. Consequently, the inhibition of ferroptosis accelerates breast cancer in lean, but not obese, mice. Obese adipocytes fail to secrete 9S-HODE, suggesting that the loss of 9S-HODE significantly contributes to the acceleration of breast cancer in obesity. Further, 9S-HODE supplementation into tumors in obese mice is sufficient to reduce tumor burden and additionally can inhibit the growth of patient-derived breast cancer organoids, underscoring its potential as a therapeutic agent.
Project description:Interventions: different phenotypes vs. HNPCC group vs. healthy control group:Nil
Primary outcome(s): Alpha diversity analysis;ß-diversity analysis;LDA EffectSize (LEfSe) analysis
Study Design: Factorial
Project description:To explore the bacterial community profile of the gut of the African palm weevil and to identify the abundance and diversity of lignin degradation-associated bacteria in each gut segment.
Project description:To compare secretory signatures of wildtype and mutant mice, the transcription profiles of tdTom+ cells from Atoh1(WT)CreERT2 R26RtdTom and Atoh1(9S/T-A)CreERT2 R26RtdTom mice were generated. The profiles were obtained for both small intestine (SI) and colon.
Project description:Bacterial DNA was detected in both subgingival and carotid plaque samples. The microbial composition differed between subgingival and carotid plaque communities. Alpha diversity analysis revealed significantly higher diversity in subgingival samples compared to carotid plaques (p = 0.039). Beta diversity analysis, including unweighted UniFrac analysis (p<0.001), linear discriminant analysis with effect size estimation, cladogram-based analysis, and principal component analysis, confirmed marked differences between the subgingival and carotid plaque microbiota. However, the study did reveal overlaps in a few individual cases.
