Project description:RNA-seq analysis of Pseudomonas sp OST1909 exposed to various preparations of naphthenic acids samples led to the identiifcation of many NA-induced genes.
Project description:The pyrite mine of Lousal, Grândola, Portugal, was discovered in 1882 and extensively exploited from 1900 until its closure in 1988 when the mining ores were not viable economically. Aiming at becoming a cultural-touristic center, the mining area was rehabilitated raising a Mining Museum and a Science Centre along with the reopening of a mining gallery for public access. In recent times, aerobiological studies on subterranean environments such as caves, mines and archeological necropolis revealed to be an important source of novel bacteria, from which their biotechnological potential are under study. Here we present a Pseudomonas sp. isolated from the mine air with potential secondary metabolite biosynthesis, resistance to antibiotics and virulence factors.
Project description:The whole proteome analysis of the Pseudomonas sp. FIP_A4 strain in presence and absence of fipronil was conducted to evaluate the differentially expressed enzymes that can play role in fipronil degradation.
Project description:Bacterial antimicrobial compounds and global regulatory networks are typically studied as separate systems, limiting our understanding of how these functions might be integrated. Here we reveal a dual-function system in the biocontrol strain Pseudomonas sp. MUP55, where the pvf cluster simultaneously functions as a global regulator of specialized metabolism and produces direct antimicrobial compounds. Metabolomic and transcriptomic analysis of a ∆pvfC mutant showed extensive global change of the regulation of metabolites and gene expression, with effects on specialized metabolites. Remarkably, pvfC differentially regulates dual siderophore systems and uncouples typically co-regulated small regulatory RNAs in the Gac/Rsm cascade. Heterologous expression confirmed the pvf cluster produces compounds with direct antimicrobial activity independent of its regulatory functions. Comparative genomic analysis revealed the MUP55 pvf cluster contains a rare additional pvfE gene found in only 3.4% of identified pvf clusters. This evolutionary integration of regulatory and defensive functions within a single genetic system provides an efficient strategy for bacterial competitive fitness and resource allocation, expanding our understanding of how beneficial microbes coordinate cellular processes while maintaining environmental competitiveness