Project description:FACS-sorted CR2+ and CR2- naive and memory CD4 T cells, plus CR2+ naive CD4 T cells activated with CD3/CD28 beads, were analyzed ex-vivo on a NanoString Immune Expression Profiling panel.
Project description:FACS-sorted CR2+ and CR2- naive and memory CD4+ T cells from healthy donors, plus CR2+ naive CD4+ T cells activated using CD3/CD28 beads, were analyzed ex-vivo. Exome-enriched RNA sequencing was carried out for all subsets.
Project description:We measured abundances of tRNAs by means of hydro-tRNA-seq (Gogakos et al., 2017), a method based on partial alkaline RNA hydrolysis that generates fragments suitable for sequencing, in the genome-reduced bacterium Mycoplasma pneumoniae.