Project description:Conventional cellular transcriptome profiling (RNA-seq) data reports “steady-state” transcript abundance rather than actual transcription rates in the nucleus. It also lacks sensitivity to detect unstable and low-abundance RNA species. We generated these maize (Zea mays L.) B73 total cellular RNA samples, extracted directly from frozen 1-mm root tips, as a parallel experiment to compare to two other types of RNA extracted from isolated nuclei.
Project description:These maize (Zea mays L.) B73 cellular RNA samples were prepared from seedlings treated in 2 h water and extracted cellular RNA directly from frozen 1-mm root tips. They are parallel experiment to compare to two other types of RNA extracted from isolated nuclei.
