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ABSTRACT:
PROVIDER: PRJNA1039532 | ENA |
REPOSITORIES: ENA
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Project description:Saururus chinensis Transcriptome or Gene expression
| PRJNA323685 | ENA
Project description:Sauchinone, a phenolic lignan derived from Saururus chinensis, possesses potent antioxidant and anti-inflammatory activities. Ultraviolet B (UVB) irradiation is a major environmental factor contributing to skin photoaging, primarily through the induction of oxidative stress and disruption of redox homeostasis in dermal fibroblasts. However, the transcriptional mechanisms underlying the protective effects of sauchinone against UVB-induced damage remain unclear. Human foreskin fibroblasts (HFFs) were treated with UVB irradiation in the presence or absence of sauchinone to investigate transcriptional changes, followed by RNA sequencing (RNA-seq) to analyze genome-wide gene expression profiles. We found that sauchinone treatment significantly reshaped the transcriptional landscape of UVB-exposed fibroblasts. KEGG pathway enrichment analysis revealed that differentially expressed genes were prominently enriched in signaling pathways related to inflammation, cellular stress responses, and extracellular signaling, including cytokine–cytokine receptor interaction, MAPK signaling pathway, and neuroactive ligand–receptor interaction. In addition, pathways associated with metabolic regulation and cellular communication were also affected. Notably, several genes involved in antioxidant defense and redox regulation, including downstream targets of the Nrf2 pathway, were upregulated under UVB + sauchinone conditions. These findings suggest that sauchinone exerts protective effects against UVB-induced oxidative stress at the transcriptional level and highlight its potential as a candidate antioxidant compound for mitigating photoaging-related cellular damage.
2026-05-27 | GSE326951 | GEO
Project description:Manassantin A is a natural product that has been isolated from the perennial herb Saururus chinensis Baill and the aquatic plant Saururus cernuus. Manassantin A has been shown to possess potent hypoxia inducible factor 1 alpha (HIF-1α) inhibitory activity in a cell-based assay screen of thousands of natural products. Manassantin A holds promise as an anti-cancer drug since it has been shown to selectively target tumor cells over normal cells. Due to the complex biological pathways involved in cancer and hypoxia, it is difficult to determine the mode-of-action by which manassantin A inhibits HIF-1. While some of the biological activities of manassantin A have been discovered in various cell-based activity assays, the molecular basis of manassantin A’s biological activities is not well characterized. The proteins in a hypoxic MDA-MB-231 cell lysate were screened for interactions with manassantin A using large scale experiments to uncover novel manassantin A interactions that lead to the drug’s HIF-1 inhibition and anti-cancer activity. Two energetics-based approaches were utilized in this manassantin A mode-of-action study: iTRAQ-SPROX and SILAC-Pulse Proteolysis. In these energetics-based approaches, protein stability is measured using the chemical denaturant dependence of either a methionine oxidation reaction (iTRAQ-SPROX) or a thermolysin protease digest (SILAC-Pulse Proteolysis). Using boh of these techniques, the stability of proteins in the absence and presence of excess manassantin A was monitored to assess ligand-induced protein stability changes.
2016-07-12 | PXD002304 | Pride
Project description:Manassantin A is a natural product that has been isolated from the perennial herb Saururus chinensis Baill and the aquatic plant Saururus cernuus. Manassantin A has been shown to possess potent hypoxia inducible factor 1 alpha (HIF-1α) inhibitory activity in a cell-based assay screen of thousands of natural products. Manassantin A holds promise as an anti-cancer drug since it has been shown to selectively target tumor cells over normal cells. Due to the complex biological pathways involved in cancer and hypoxia, it is difficult to determine the mode-of-action by which manassantin A inhibits HIF-1. While some of the biological activities of manassantin A have been discovered in various cell-based activity assays, the molecular basis of manassantin A’s biological activities is not well characterized. The proteins in a hypoxic MDA-MB-231 cell lysate were screened for interactions with manassantin A using large scale experiments to uncover novel manassantin A interactions that lead to the drug’s HIF-1 inhibition and anti-cancer activity. Two energetics-based approaches were utilized in this manassantin A mode-of-action study: iTRAQ-SPROX and SILAC-Pulse Proteolysis. In these energetics-based approaches, protein stability is measured using the chemical denaturant dependence of either a methionine oxidation reaction (iTRAQ-SPROX) or a thermolysin protease digest (SILAC-Pulse Proteolysis). Using boh of these techniques, the stability of proteins in the absence and presence of excess manassantin A was monitored to assess ligand-induced protein stability changes.
2016-07-19 | PXD002305 | Pride
Project description:Pistacia chinensis Bunge is known as dioecious, but we have found wild monoecious individuals. In order to screen the candidate genes which may influence the sex expression or floral phenotypic differences of P. chinensis, the inflorescence buds for different sex types associated with the sex differentiation were selected and tested for small RNA sequencing. Sex-specific differentially expressed small RNA were discovered, combined with real-time PCR data, the regulation patterns of various sex types were first revealed. Our study represents the first detailed analysis of small RNA sequencing, providing more clues for understanding the mechanism of sex determination on P. chinensis.
2020-01-01 | GSE128023 | GEO