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ABSTRACT:
PROVIDER: PRJNA1039595 | ENA |
REPOSITORIES: ENA
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Project description:Analysis of microbial gene expression in response to physical and chemical gradients forming in the Columbia River, estuary, plume and coastal ocean was done in the context of the environmental data base. Gene expression was analyzed for 2,234 individual genes that were selected from fully sequenced genomes of 246 prokaryotic species (bacteria and archaea) as related to the nitrogen metabolism and carbon fixation. Seasonal molecular portraits of differential gene expression in prokaryotic communities during river-to-ocean transition were created using freshwater baseline samples (268, 270, 347, 002, 006, 207, 212).
2010-09-10 | GSE18303 | GEO
Project description:Analysis of microbial gene expression in response to physical and chemical gradients forming in the Columbia River, estuary, plume and coastal ocean was done in the context of the environmental data base. Gene expression was analyzed for 2,234 individual genes that were selected from fully sequenced genomes of 246 prokaryotic species (bacteria and archaea) as related to the nitrogen metabolism and carbon fixation. Seasonal molecular portraits of differential gene expression in prokaryotic communities during river-to-ocean transition were created using freshwater baseline samples (268, 270, 347, 002, 006, 207, 212). Total RNA was isolated from 64 filtered environmental water samples collected in the Columbia River coastal margin during 4 research cruises (14 from August, 2007; 17 from November, 2007; 18 from April, 2008; and 16 from June, 2008), and analyzed using microarray hybridization with the CombiMatrix 4X2K format. Microarray targets were prepared by reverse transcription of total RNA into fluorescently labeled cDNA. All samples were hybridized in duplicate, except samples 212 and 310 (hybridized in triplicate) and samples 336, 339, 50, 152, 157, and 199 (hybridized once). Sample location codes: number shows distance from the coast in km; CR, Columbia River transect in the plume and coastal ocean; NH, Newport Hydroline transect in the coastal ocean at Newport, Oregon; AST and HAM, Columbia River estuary locations near Astoria (river mile 7-9) and Hammond (river mile 5), respectively; TID, Columbia River estuary locations in the tidal basin (river mile 22-23); BA, river location at Beaver Army Dock (river mile 53) near Quincy, Oregon; UP, river location at mile 74.
2010-09-10 | E-GEOD-18303 | biostudies-arrayexpress
Project description:Soil microbial diversity of ultramafic substrates in New Caledonia
| PRJNA343044 | ENA
Project description:Nematoda of New Caledonia
| PRJEB22114 | ENA
Project description:An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal. Human monocyte derived dendritic cells (DCs) were infected either with chicken egg grown A/California/7/2009, A/Brevig Mission/1/1918, A/New Caledonia/20/99or A/Texas/36/91. Samples were taken and fixed in RNA stabilizing regaent at 2, 2.7, 3.3, 4, 5, 6, 7 and 8 hours. Cells exposed to chicken egg alantoic fluid served as a control. Infections with A/California/7/2009, A/New Caledonia/20/99 or A/Texas/36/91 were carried out at a BSL2 enviroment. Infections with A/Brevig Mission/1/1918 were carried out in a BSL3 enviroment in parralel to the BSL2 infections with cells from the same donor and cell preparation.
2015-08-20 | E-GEOD-55277 | biostudies-arrayexpress
Project description:An 8 hours timecourse was performed with human DCs infected either with A/California/7/2009 and A/Brevig Mission/1/1918 (pandemic) or A/New Caledonia/20/99 and A/Texas/36/91 seosonal. Human monocyte derived dendritic cells (DCs) were infected either with chicken egg grown A/California/7/2009, A/Brevig Mission/1/1918, A/New Caledonia/20/99or A/Texas/36/91. Samples were taken and fixed in RNA stabilizing regaent at 2, 2.7, 3.3, 4, 5, 6, 7 and 8 hours. Cells exposed to chicken egg alantoic fluid served as a control. Infections with A/California/7/2009, A/New Caledonia/20/99 or A/Texas/36/91 were carried out at a BSL2 enviroment. Infections with A/Brevig Mission/1/1918 were carried out in a BSL3 enviroment in parralel to the BSL2 infections with cells from the same donor and cell preparation.
2015-08-20 | E-GEOD-55276 | biostudies-arrayexpress