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ABSTRACT: CERI-KRISP Influenza Sequencing in South Africa

PROVIDER: PRJNA1040207 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR26824811_1.fastq.gz	Fastqsanger.gz
	SRR26824811_2.fastq.gz	Fastqsanger.gz
	SRR26824812_1.fastq.gz	Fastqsanger.gz
	SRR26824812_2.fastq.gz	Fastqsanger.gz
	SRR26824813_1.fastq.gz	Fastqsanger.gz

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Real-time quantitative PCR analysis of mouse lungs after exposure to SARS-CoV-2 and variants of concern (VOC)

Project description:Age-matched K18-hACE2 transgenic mice were infected intranasally with different SARS-CoV-2 viruses, including (1) USA-WA1/2020 (WA) of lineage A, (2) New York-PV09158/2020 (NY) of lineage B.1.3, (3) USA/CA_CDC_5574/2020 (CA) of lineage B.1.1.7 and (4) hCoV-19/South Africa/KRISP-EC-K005321/2020 (SA) of lineage B.1.351. Mouse lungs were harvested on 3 days post infection (dpi). Total RNA was extracted using QIAgen RNeasy Plus Mini Kit and was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Resultant cDNA was used as the template along with RT2 SYBR Green ROX qPCR Mastermix (Qiagen) to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system.

2021-10-10 | GSE183621 | GEO

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