Candida albicans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA104145 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We have used DNA microarray technology to compare the expression of over 6000 genes between C. albicans WT and ACE2 knockout strains in the yeast form. Keywords: Gene expression comparison
2007-02-23 | GSE7099 | GEO
Project description:RNA-Seq of Candida albicans WT vs zcf13 mutant
| PRJNA1074308 | ENA
Project description:The Regulation of Ace2 and Morphogenesis (RAM) pathway is an important regulatory network in the human fungal pathogen Candida albicans. The RAM pathway’s two most well-studied components, the NDR/Lats kinase Cbk1 and its putative substrate, the transcription factor Ace2, have a wide range of phenotypes and functions. It is not clear, however, which of these functions are specifically due to the phosphorylation of Ace2 by Cbk1. To address this question, we first compared the transcriptional profiles of CBK1 and ACE2 deletion mutants. This analysis indicates that, of the large number of genes whose expression is affected by deletion of CBK1 and ACE2, only 5.5% of those genes are concordantly regulated. Our data also suggest that Ace2 directly or indirectly represses a large set of genes during hyphal morphogenesis. Second, we generated strains containing ACE2 alleles with alanine mutations at the Cbk1 phosphorylation sites. Phenotypic and transcriptional analysis of these ace2 mutants indicates that, as in Saccharomyces cerevisiae, Cbk1 regulation is important for daughter cell localization of Ace2 and cell separation during yeast phase growth. In contrast, Cbk1 phosphorylation of Ace2 plays a minor role in C. albicans yeast-to-hyphae transition. We have, however, discovered a new function for the Cbk1-Ace2 axis. Specifically, Cbk1 phosphorylation of Ace2 prevents the hyphae-to-yeast transition. To our knowledge, this is one of the first regulators of the C. albicans hyphae-to-yeast transition to be described. Finally, we present an integrated model for the role of Cbk1 in the regulation of hyphal morphogenesis in C. albicans.
2020-08-26 | GSE155450 | GEO
Project description:Candida albicans TRKO rbf1Δ/hfl1Δ/dpb4Δ vs WT transcriptome data
| PRJNA234493 | ENA
Project description:Candida wildtype cells control vs. Candida QDR1/QDR2/QDR3 knockout cells test
| PRJNA177378 | ENA
Project description:mRNA expression profile for BMDMs from wildtype and JNK1 knockout mice stimulated with yeast form Candida albicans
2017-01-18 | GSE83265 | GEO
Project description:Ace2 transcription factor family genes are found in many fungal genomes and are required for regulation of expression of genes involved in cell separation. We used transcriptional profiling to identify the targets of Ace2 in Candida albicans, and we show that these include several cell wall components, such as glucanases and glycosylphosphatidylinositol-anchored proteins. Expression is downregulated in ace2 deletion mutants in both yeast and hyphal cells. In addition, deleting ace2 results in dramatic changes in expression of metabolic pathways. Expression of glycolytic enzymes is reduced, while expression of respiratory genes (including those involved in the tricarboxylic acid cycle, oxidative phosphorylation, and ATP synthesis) is increased. Similar changes occur in both yeast and hyphal cells. In contrast, genes required for acetyl-coenzyme A and lipid metabolism are upregulated in an ace2 deletion mutant grown predominantly as yeast cells but are downregulated in hyphae. These results suggest that in wild-type strains, Ace2 acts to increase glycolysis and reduce respiration. This is supported by the observation that deleting ace2 results in increased resistance to antimycin A, a drug that inhibits respiration. We also show that Ace2 is required for filamentation in response to low oxygen concentrations (hypoxia). We suggest that filamentation is induced in wild-type cells by reducing respiration (using low oxygen or respiratory drugs) and that mutants with increased respiratory activity fail to undergo filamentation under these conditions. This SuperSeries is composed of the SubSeries listed below.
2007-02-23 | GSE7105 | GEO
Project description:Transcriptional profiling of Candida cells vs putative MFS transporter QDR1 knockout cells One-condition experiment, WT vs. QDR1 knockout cells. Biological replicates: 3 control, 3 treated, independently grown and harvested.
2013-03-01 | E-GEOD-41531 | biostudies-arrayexpress
Project description:Transcriptional profiling of Candida cells vs putative MFS transporter QDR1 knockout cells One-condition experiment, WT vs. QDR3 knockout cells. Biological replicates: 3 control, 3 treated, independently grown and harvested.
2013-03-01 | E-GEOD-41533 | biostudies-arrayexpress