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RFX6 maintains gene expression and function of adult human islet ⍺ cells [RNAseq]

ABSTRACT: RFX6 maintains gene expression and function of adult human islet ⍺ cells [RNAseq]

PROVIDER: PRJNA1046114 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26974573_1.fastq.gz	Fastqsanger.gz
	SRR26974573_2.fastq.gz	Fastqsanger.gz
	SRR26974574_1.fastq.gz	Fastqsanger.gz
	SRR26974574_2.fastq.gz	Fastqsanger.gz
	SRR26974575_1.fastq.gz	Fastqsanger.gz

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RFX6 maintains gene expression and function of adult human islet ⍺ cells

Project description:RFX6 maintains gene expression and function of adult human islet ⍺ cells

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RFX6 maintains gene expression and function of adult human islet ⍺ cells [CUT&RUN]

Project description:RFX6 maintains gene expression and function of adult human islet ⍺ cells [CUT&RUN]

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RFX6 maintains gene expression and function of adult human islet ⍺ cells [RNAseq]

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RFX6 maintains gene expression and function of adult human islet ⍺ cells [CUT&RUN]

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Identification of Rfx6-dependent genes in the adult mouse ileon by RNAseq

Project description:The goal of this experiment was to identify Rfx6-regulated genes in the ileum of adult mice by comparative transcriptome studies, 8 days and 3 months after Rfx6 removal .

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Identification of Rfx6-dependent genes in the adult mouse ileon by RNAseq

Project description:Identification of Rfx6-dependent genes in the adult mouse ileon by RNAseq

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Genes regulated by the winged helix transcription factor Rfx6 in adult beta cell in the mouse

Project description:The goal of this study was to identify genes which are differentiatlly expresesd upon induced inactivation of Rfx6 in beta cell in adult mice

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Rfx6 target genes in the Min6b1 cell line, a pancreatic beta cell model

Project description:Increasing evidence suggests that loss of beta cell characteristics may cause insulin secretory deficiency in diabetes but the underlying mechanisms remain unclear. Here we show that Rfx6, whose mutation leads to neonatal diabetes in man, is essential to maintain key features of functionally mature beta cells in mice. Rfx6 loss in adult beta cells leads to glucose intolerance, impaired beta cell glucose sensing and to defective insulin secretion. This is associated with reduced expression of core components of the insulin secretion pathway including GLucokinase, the Abcc8/SUR1 subunit of KATP channels and voltage-gated Ca2 channels, which are direct targets of Rfx6. Moreover, Rfx6 contributes to the silencing of the vast majority of “disallowed” genes, a group usually specifically repressed in adult beta cells, and thus to the maintenance of beta cell maturity. These findings raise the possibility that changes in Rfx6 expression or activity may contribute to beta cell failure in man.

2014-12-11 | GSE62844 | GEO

PDX1+ cell budding morphogenesis in a stem cell-derived islet spheroid system

Project description:Remarkable advancements in protocol development have been achieved to manufacture insulin-secreting islets from human pluripotent stem cells. Distinct from current approaches, we devised a tunable strategy to generate islet spheroids enriched for major islet cell types by incorporating PDX1+ cell budding morphogenesis into the differentiation process of staged transcriptional programming. In this process that appears to mimic normal islet morphogenesis, the differentiating islet spheroids self-organize with endocrine cells that are intermingled or arranged in a core-mantle architecture, accompanied with functional heterogeneity. Through in vitro modelling of human pancreas development, we illustrate the importance of PDX1 in eliciting cell budding morphogenesis, and the requirement for EphB3/4 signaling. We show how RFX6 deficiency affects pancreatic patterning and uncover an expected role of RFX6 in early pancreas morphology. The tunable differentiation system and stem cell-derived islet models described in this work may facilitate addressing fundamental questions in islet biology and probing human pancreas diseases.

2024-05-15 | GSE249020 | GEO

Rfx6 target genes in the Min6b1 cell line, a pancreatic beta cell model

Project description:Increasing evidence suggests that loss of beta cell characteristics may cause insulin secretory deficiency in diabetes but the underlying mechanisms remain unclear. Here we show that Rfx6, whose mutation leads to neonatal diabetes in man, is essential to maintain key features of functionally mature beta cells in mice. Rfx6 loss in adult beta cells leads to glucose intolerance, impaired beta cell glucose sensing and to defective insulin secretion. This is associated with reduced expression of core components of the insulin secretion pathway including GLucokinase, the Abcc8/SUR1 subunit of KATP channels and voltage-gated Ca2 channels, which are direct targets of Rfx6. Moreover, Rfx6 contributes to the silencing of the vast majority of M-bM-^@M-^\disallowedM-bM-^@M-^] genes, a group usually specifically repressed in adult beta cells, and thus to the maintenance of beta cell maturity. These findings raise the possibility that changes in Rfx6 expression or activity may contribute to beta cell failure in man. Two ChIP-Seq experiments have been performed : 1) anti-HA ChIP-Seq on 3HA-Rfx6 transfected Min6b1 cells and 2) anti-HA ChIP-Seq on Min6b1 cells

2014-12-11 | E-GEOD-62844 | biostudies-arrayexpress

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