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ABSTRACT: Exploring the microbial mechanism of the negative effects of amoxicillin and copper on the humification of aerobic composting of dairy manure

PROVIDER: PRJNA1047967 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27068100_1.fastq.gz	Fastqsanger.gz
	SRR27068100_2.fastq.gz	Fastqsanger.gz
	SRR27068077_1.fastq.gz	Fastqsanger.gz
	SRR27068077_2.fastq.gz	Fastqsanger.gz
	SRR27068078_1.fastq.gz	Fastqsanger.gz

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Project description:Microbial mechanisms involved in the negative effects of amoxicillin and copper on humification during composting of dairy cattle manure

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Transcriptome analysis of potato tubers: Effects of different agricultural practices

Project description:The use of profiling techniques such as transcriptomics, proteomics, and metabolomics has been proposed to improve the detection of side effects of plant breeding processes. This paper describes the construction of a food safety-oriented potato cDNA microarray (FSPM). Microarray analysis was performed on a well-defined set of tuber samples of two different potato varieties, grown under different, well-recorded environmental conditions. Data were analyzed to assess the potential of transcriptomics to detect differences in gene expression due to genetic differences or environmental conditions. The most pronounced differences were found between the varieties Sante and Lady Balfour, whereas differences due to growth conditions were less significant. Transcriptomics results were confirmed by quantitative PCR. Furthermore, the bandwidth of natural variation of gene expression was explored to facilitate biological and/or toxicological evaluation in future assessments. Keywords: experiment with factorial design factorial design; 2 potato cultivars (Sante, Lady Balfour); 2 fertilizers (dairy manure compost, chicken manure pellets); 3 plant protection treatments (copper oxychloride, comcat, water), 3 biological replicates, 48 samples

2009-02-01 | E-GEOD-11875 | biostudies-arrayexpress

Protein expression profile in composting habitat

Project description:Metaproteomics analysis of the evolution of protein expression profile during cattle manure composting

2025-01-07 | PXD059527 |

Project description:Microbial characteristics and safety of dairy manure composting for reuse as dairy bedding

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Proteomic investigation of a mixed microbial culture synthesizing polyhydroxyalkanoates from fermented dairy manure under aerobic feast-famine conditions

Project description:Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures. To maximize PHA production, mixed microbial cultures may be enriched for PHA-producing bacteria with a high storage capacity through the imposition of cyclic, aerobic feast-famine conditions in a sequencing batch reactor (SBR). Though enrichment SBRs have been extensively investigated a bulk solutions-level, little evidence at the proteome level is available to describe the observed SBR behavior to guide future SBR optimization strategies. As such, the purpose of this investigation was to characterize proteome dynamics of a mixed microbial culture in an SBR operated under aerobic feast-famine conditions using fermented dairy manure as the feedstock for PHA production. At the beginning of the SBR cycle, excess PHA precursors were provided to the mixed microbial culture (i.e., feast), after which followed a long duration devoid of exogenous substrate (i.e., famine). Two-dimensional electrophoresis was used to separate protein mixtures during a complete SBR cycle, and proteins of interest were identified.

2017-01-31 | PXD003126 | Pride

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