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TimeLapse-seq after amino acid starvation in Mettl3 knockout MEF

ABSTRACT: TimeLapse-seq after amino acid starvation in Mettl3 knockout MEF

PROVIDER: PRJNA1048352 | ENA |

REPOSITORIES: ENA

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TimeLapse-seq after amino acid starvation in Mettl3 knockout MEF

Project description:Degradation of mRNA containing N6-methyladenosine (m6A) is essential for cell growth, differentiation, and stress responses. Here, we show that m6A markedly alters ribosome dynamics and that these alterations mediate the degradation effect of m6A on mRNA. We find that m6A is a potent inducer of ribosome stalling, and these stalls lead to ribosome collisions that form a unique conformation unlike those seen in other contexts. We find that the degree of ribosome stalling correlates with m6A-mediated mRNA degradation, and increasing the persistence of collided ribosomes correlates with enhanced m6A-mediated mRNA degradation. Ribosome stalling and collision at m6A is followed by recruitment of YTHDF m6A reader proteins to promote mRNA degradation. We show that mechanisms that reduce ribosome stalling and collisions, such as translation suppression during stress, stabilize m6A-mRNAs and increase their abundance, enabling stress responses. Overall, our study reveals the ribosome as the initial m6A sensor for beginning m6A-mRNA degradation.

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AmpliSeq Data of WT and SLC38A10 knockout primary cortex cells in amino acid starvation and refed conditions

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Ribosome profiling and parallel RNA-seq of S. pombe cells subject to amino acid starvation

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RNA changes upon glucose starvation

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