Project description:Allogeneic transplant of cells/tissue/organ from a genetically dissimilar donor can lead to severe complications that are largely immune-mediated. We used gene expression data from microarrays to understand how allo-stimulation provided by irradiated peripheral blood mononuclear cells (PBMC) (stimulators) differentially affect the functionality of CD19+ B cells of responder PBMC across different responder-stimulator donor pairs.
Project description:RNAseq analysis of BAFF in vitro-stimulated (6 hours) murine nfkb2fl/flCD19-Cre (furtheron designated as REL) and CD19-Cre (furtheron designated as WT) splenic B cells identifies genes regulated by the transcription factor NF-kB2 in BAFF-stimulated B cells.