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ABSTRACT: Edwardsiella LD

PROVIDER: PRJNA1051295 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27180880.fastq.gz	Fastqsanger.gz
	SRR27180881.fastq.gz	Fastqsanger.gz
	SRR27180882.fastq.gz	Fastqsanger.gz
	SRR27180883.fastq.gz	Fastqsanger.gz
	SRR27180884.fastq.gz	Fastqsanger.gz

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LD PROTEOMICS WITH TURBOT INFECTED BY EIB202

Project description:To investigate the impact of pathogenic immune stimulation on the lipid droplet (LD) proteome of turbot (Scophthalmus maximus), turbot were intraperitoneally injected with PBS (XA01292LQ_P), wild-type Edwardsiella piscicida EIB202 (XA01292LQ_W), or inactivated E. piscicida EIB202 (XA01292LQ_T). Liver tissues were collected, and LDs were extracted for 4D label-free quantitative proteomics analysis.

2024-11-21 | PXD047605 | Pride

Expression analysis of channel catfish anterior kidney at 48h post immersion challenge of vaccinated fish (28 days post vaccination with attenuated Edwardsiella ictaluri) with virulent E. ictaluri

2011-10-05 | GSE32588 | GEO

Quantitative Analysis of ZF4 cells Transcriptomes when Infected with Edwardsiella tarda

Project description:Edwardsiella tarda is a Gram-negative bacterial pathogen that can infect a wide range of freshwater and marine fish. However, the immune evasion mechanisms of Edwardsiella tarda is not fully understood. We found that Edwardsiella tarda infection generally significantly upregulated and downregulated a lot of immune-related genes of zebrafish ZF4 cells using RNA-seq technology.

2017-02-14 | GSE81773 | GEO

Expression analysis of channel catfish anterior kidney at 48h post immersion vaccination with attenuated Edwardsiella ictaluri

Project description:To determine the global gene transcriptional changes in channel catfish anterior kidney after immersion vaccination with attenuated Edwardsiella ictaluri compared to sham vaccinated catfsih control. The attenuated Edwardsiella ictaluri used for vaccination was Aquavac-ESC which was commercially available.

2011-09-10 | GSE32046 | GEO

Gene expression profiles of vaccinated fish using a long-term efficiency mixed vaccine against Edwardsiella tarda performed by a new designed oligo DNA microarray in Japanese flounder Paralichthys olivaceus

Project description:Gene expression profiles by microarray have contributed for a elucidation of an immune-response and a determination of efficiency in vaccination. Recent day, edwardsielosis have caused a fatal damage in the aquaculture of Japanese flounder, Paralichthys olivaceus. However the formalin killed-cell vaccines made from Edwardsiella tarda isolated same fish species were not efficient. Recent our study revealed the mixed FKC vaccine made from the two different type of E. tarda protected Japanese flounder against Edwardsiella tarda infection for long-term. In this study, we analyzed the immune-response of a vaccinated fish kidney using the mixed FKC vaccine against Edwardsiella tarda with an Agilent custom-oligo DNA microarray on 9,573 probes of Japanese flounder. Our study revealed that the mixed FKC vaccine confered a strong immune-response and keeped a efficient for long-term on Japanese flounder.

2014-12-31 | GSE26808 | GEO

Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection

Project description:This SuperSeries is composed of the following subset Series: GSE28481: Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection using a static immersion systems [experiment A] GSE28485: Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection [experiment B] Refer to individual Series

2011-09-30 | E-GEOD-28486 | biostudies-arrayexpress

Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection [experiment B]

Project description:Transcriptional profiling of the zebrafish embryonic host response to infection by injection of 200 CFUs of Edwardsiella tarda (strain FL6-60)

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Expression analysis of channel catfish anterior kidney at 48h post immersion challenge of vaccinated fish (28 days post vaccination with attenuated Edwardsiella ictaluri) with virulent E. ictaluri

Project description:To determine the global gene transcriptional changes in vaccinated channel catfish anterior kidney after immersion challenge with a virulent Edwardsiella ictaluri compared to sham vaccinated catfish control. The attenuated Edwardsiella ictaluri used for vaccination was Aquavac-ESC which was commercially available, the virulent strain of Edwardsiella ictaluri is the AL93-58 strain. A six chip study using total RNA samples including 3 controls (no vaccination, no challenge) and 3 treated groups (challenge after vaccination). Each RNA sample used for microarray was pooled fish anterior kidney samples (5 fish anterior kidney samples were pooled as one). Each chip measrues the expression level of 65,182 genes. For each sequence, 3 probes were selected. Two copies were prepared on arrays.

2011-10-04 | E-GEOD-32588 | biostudies-arrayexpress

ArgR-dependent Bacterial Resistance to Host Lipid Droplets in Edwardsiella piscicida

Project description:Lipid droplets (LDs), as innate immune hubs, function in the front line of antimicrobial defense involved in the ongoing host-pathogen arms race. Particularly for intracellular bacterial pathogens, the endowed capacity to resist host LDs can effectively facilitate pathogen in vivo colonization and evasion from the host’s innate immune response. Here, to investigate the genetic mechanisms of intracellular bacteria response to host LDs, we utilized transposon insertion sequencing to dissect critical fitness determinants of Edwardsiella piscicida under the treatment of LDs isolated from its native host, turbot. Targeted metabolomics indicated that LD challenge resulted in the accumulation of intracellular arginine. The core arginine metabolism regulatory factor, ArgR, was found to play a pivotal role in combating LDs, emphasizing the importance of orchestrating intracellular arginine levels for bacterial LD adaptation. Specifically, ArgR enhanced the expressions of genes involved in arginine catabolism (speA/B and arcC) and diminished gene transcripts associated with arginine import (artP) and synthesis (argD/E/H). Furthermore, ArgR contributed to the pathogenesis of E. piscicida, promoting the proliferation in host cells and virulence in turbot. Collectively, our results shed light on the underlying mechanism of intracellular pathogens resisting LDs during bacterial infections and highlighting the crucial role of arginine in the host-pathogen interactions.

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Expression analysis of Edwardsiella ictaluri 93-146 wild type and mutant strains during catfish fry encounter.

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