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Epigenetic regulation in small intestinal epitheliel cells with butyrate exposure

ABSTRACT: Epigenetic regulation in small intestinal epitheliel cells with butyrate exposure

PROVIDER: PRJNA1051497 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27184704_1.fastq.gz	Fastqsanger.gz
	SRR27184704_2.fastq.gz	Fastqsanger.gz
	SRR27184705_1.fastq.gz	Fastqsanger.gz
	SRR27184705_2.fastq.gz	Fastqsanger.gz
	SRR27184706_1.fastq.gz	Fastqsanger.gz

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Epigenetic regulation in small intestinal epitheliel cells with butyrate exposure

Project description:H3K9Ac enrichment in intestinal epithelial cells from the intestine of WT mice treated with vehicle or buytrate for 7 days. Intestinal epithelial cells were harvested from the terminal ileum of vehicle or butyrate treated mice. Chromatin immunoprecipitation was performed with anti-H3K9Ac. Sequencing was performed using the Illumina and reads were mapped to mm10 genome using STAR Aligner.

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Differential gene expression in iPSC-derived human intestinal epithelial cell layers following exposure to two concentrations of the Short Chain Fatty Acids butyrate, propionate and acetate

Project description:Intestinal epithelial cells and the intestinal microbiota are in a mutualistic relationship that is dependent on communication. This communication is multifaceted, but one aspect is communication through compounds produced by the microbiota such as the short-chain fatty acids (SCFAs) butyrate, propionate and acetate. Studying the effects of SCFAs and especially butyrate in intestinal epithelial cell lines like Caco-2 cells has been proven problematic. In contrast to the in vivo intestinal epithelium, Caco-2 cells do not use butyrate as an energy source, leading to a build-up of butyrate. Therefore, we used human induced pluripotent stem cell derived intestinal epithelial cells, grown as a cell layer, to study the effects of butyrate, propionate and acetate on whole genome gene expression in the cells. For this, cells were exposed to concentrations of 1 and 10 mM of the individual short-chain fatty acids for 24 hours. Unique gene expression profiles were observed for each of the SCFAs in a concentration-dependent manner. Evaluation on both an individual gene level and pathway level showed that butyrate induced the biggest effects followed by propionate and then acetate. Several known effects of SCFAs on intestinal cells were confirmed, such as effects on metabolism and immune responses. The changes in metabolic pathways in the intestinal epithelial cell layers in this study demonstrate that there is a switch in energy source from glucose to SCFAs, thus induced pluripotent stem cell derived intestinal epithelial cell are responding in a similar manner to SCFAs as in vivo intestinal tissues.

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Project description:In this experiment, we collected intestines from rat pups with the following conditions: A. Control; B. NEC; C. NEC+NaBu (sodium butyrate); D. NEC+NSC23766. This is the results of proteomics from these intestinal tissues.

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