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ABSTRACT: Transcriptome study on fish meal requirement

PROVIDER: PRJNA1051837 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27198258_1.fastq.gz	Fastqsanger.gz
	SRR27198258_2.fastq.gz	Fastqsanger.gz
	SRR27198259_1.fastq.gz	Fastqsanger.gz
	SRR27198259_2.fastq.gz	Fastqsanger.gz
	SRR27198260_1.fastq.gz	Fastqsanger.gz

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Project description:intestinal microbiota on fish meal requirement

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Effects of Lithospermum erythrorhizon on gut health and growth performance of pearl gentian grouper based on Transcriptome

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On the compatibility of fish meal replacements in aquafeeds for rainbow trout. A combined metabolomic, proteomic and histological study.

Project description:The sustainable development of modern aquaculture must rely on a significant reduction of the fish meal (FM) used in aquafeed formulations. However, FM substitution with alternative ingredients in diets for carnivorous fish species often showed reduced nutrient absorption, significantly perturbed metabolisms and histological changes at both hepatic and intestinal level. In the present study, adult rainbow trout (Oncorhynchus mykiss) were fed three different experimental aquafeed formulations. A control diet with higher FM content (27.3%) than two test formulations in which fish meal was substituted with two more sustainable and promising alternatives: insect meal (Hermetia illucens larvae=10.1%, FM=11.6%) and poultry by-products meal (PBM=14.8%; FM=11.7%). Combined metabolomics and proteomics analyses of fish liver, together with histological examination of liver and intestine demonstrated that a well balanced formulation of nutrients in the three diets allowed high metabolic compatibility of either substitutions, paving the way for innovative and sustainable use of novel raw materials for the fish feed industry. Results show that the main metabolic pathways of nutrient absorption and catabolism were essentially unaltered by alternative feed ingredients, and also histological alterations were negligible. It is demonstrated that substitution of fish meal with sustainable alternatives does not impact on fish metabolism, given proper efforts are put in fulfilling nutritional requirements of rainbow trout.

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Gene expression profiling of white bass when fed fish meal or fish meal replacement diets

Project description:White bass (Morone chrysops) are a popular sportfish throughout the southern United States, and one parent of the commercially successful hybrid striped bass (M. chrysops x M. saxatilis). Currently, white bass are cultured using diets formulated for other carnivorous fish, such as largemouth bass (Micropterus salmoides) or hybrid striped bass and contain a significant percentage of marine fish meal. Since there are no studies regarding the utilization of alternative proteins in this species, we evaluated global gene expression of white bass fed diets in which fish meal was partially or totally replaced by various combinations of soybean meal, poultry by-product meal, canola meal, soy protein concentrate, wheat gluten, or a commercial protein blend (Pro-Cision). Significant differential expressed genes and gene ontology of pairwise comparisons between control diet and each test diet are presented and discussed.

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Data on European seabass fed with methionine supplemented diets obtained through label free shotgun proteomics

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